|关键词||棉花花 黄酮类化合物 阿尔茨海默病 抗氧化|
In this paper,we investigated the material basis of total flavonoids from Flos Gossypii. and their activity of anti-Alzheimer's disease. 1. The separation and structural identification of flavonoids from Flos Gossypii. Total flavonoids of Flos Gossypii were from mature and stable process route which were done by our lab study .Eight flavonoids were separated by polyamide and sephadex LH-20 column chromatograph. The structural identifications of compounds were carried out using 1HNMR, 13CNMR and high-performance liquid chromatography multistage tandem mass spectrometry as well as comparing with our previous report. 2. Research on antioxidant activity of total flavonoids and flavonoids from Flos Gossypii. The antioxidant activities of compounds as well as total flavonoids from Flos Gossypii were examined by two tests: DPPH and ABTS radical activity. The data showed that eight compounds and total flavonoids had the antioxidant activity. We also discussed the structure-activity relationship of these compounds. 3. Research on inhibition of acetylcholinesterase (AchE) of total flavonoids and flavonoids of Flos Gossypii. AchE inhibitors were therapeutic drug of treating AD, which were still research hotspot of chemical chemistry. Some study showed that flavonoids have AchE inhibition activity. In this paper we investigated the AchE inhibition of total flavonoids and then flavonoids from Flos Gossypii and analyzed the molecular docking energy between ligands (flavonoids) and receptor (AchE). 4. Research on protection action of tBHP- induced PC12 cell injure from total flavonoids and flavonoids of Flos Gossypii. Reactive oxygen species are believed to play a very significant role in the pathogenesis of several diseases including AD. There are some studies suggesting that oxidative stress is one of the reason of AD degenerative change. In this paper, total flavonoids and flavonoids of Flos Gossypii were investigated the protection of PC12 cell injure induced by tert-Butyl hydroperoxide (tBHP). 5. Research on protection action and mechanism of H2O2-induced SK-N-BE-2 cell injure and mechanism of action from total flavonoids and flavonoids of Flos Gossypii. Bcl-2, which is a kind of apoptosis inhibiting factor, was located in mitochondria and it played pivotal role in intracellular apoptosis signal. In this paper we researched on the protection action of SK-N-BE-2 cell apoptosis from total flavonoids and flavonoids of Flos Gossypii and investigated the Bcl-2 gene expression change. 6. Research on isoquercetin standard substance Natural product standard substance is very important in verifing the quality of product during the quality control of natural product. Isoquercetin is one of component of total flavonoids from Flos Gossypii. , which has the antioxidant activity and eliminate the free radical .In present, isoquercetin has not reported in the market at home and abroad. Understanding it through research, we can take it as the standard substance to evaluate and make sure the analytical result of the quality of product accurate and legal.
|赵永昕. 棉花花总黄酮物质基础及抗阿尔茨海默病相关生物活性研究[D]. 北京. 中国科学院大学,2012.|
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