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题名: 一种用于转基因水稻快速检测的DNA提取方法
作者: 苟小清; 付振艳; 王晓军
关键词: 转基因水稻 ; DNA快速提取 ; PCR模板
刊名: 安徽农业科学
发表日期: 2012
DOI: 10.13989/j.cnki.0517-6611.2012.35.117
卷: 40, 期:35, 页:17014-17015+17018
资助者: 中国科学院“西部博士”资助项目(XBBS200914);新疆维吾尔自治区自然基金会项目(2011211B507)
摘要: [目的]建立一种快速筛选转基因水稻的DNA提取方法。[方法]对试剂盒法和快速法2种DNA提取方法进行比较,并分别用转Ta NADP-ME1和Ta NADP-ME2基因的转基因水稻进行验证。[结果]用快速法提取得到的DNA进行PCR扩增,可分别得到约1 700 bp的Ta NADP-ME2基因和约1 900 bp的Ta NADP-ME1基因,且条带清晰明亮,与试剂盒法相比无显著差异。[结论]该研究中快速提取DNA的方法经济、简便、快捷,适合用于对大量转基因样品进行检测。
英文摘要: [Objective]To establish a rapid DNA extraction method for the screening of transgenic rice.[Method]The kit method and fast methodof two DNA extraction methods were compared,and were proved by Ta NADP - ME1 and Ta NADP-ME2 gene transgenic rice respectively.[Result]PCR was done by using the DNA which extracted by the rapid method as template,about 1 700 bp Ta NADP-ME2 gene and about 1900bp Ta NADP-ME1 gene were obtained respectively,and the strips were clear and bright,comparing with the kit method showed no significantdifference.[Conclusion]The rapid DNA extraction method of this study is economic,simple and quick,suitable for the large transgenic samplesdetection.
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内容类型: 期刊论文
URI标识: http://ir.xjipc.cas.cn/handle/365002/2440
Appears in Collections:资源化学研究室_期刊论文

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作者单位: 中国科学院新疆理化技术研究所;中国科学院研究生院

Recommended Citation:
苟小清,付振艳,王晓军. 一种用于转基因水稻快速检测的DNA提取方法[J]. 安徽农业科学,2012,40(35):17014-17015+17018.
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文件名: 一种用于转基因水稻快速检测的DNA提取方法.pdf
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