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苟小清; 付振艳; 王晓军
Source Publication安徽农业科学
Abstract[目的]建立一种快速筛选转基因水稻的DNA提取方法。[方法]对试剂盒法和快速法2种DNA提取方法进行比较,并分别用转Ta NADP-ME1和Ta NADP-ME2基因的转基因水稻进行验证。[结果]用快速法提取得到的DNA进行PCR扩增,可分别得到约1 700 bp的Ta NADP-ME2基因和约1 900 bp的Ta NADP-ME1基因,且条带清晰明亮,与试剂盒法相比无显著差异。[结论]该研究中快速提取DNA的方法经济、简便、快捷,适合用于对大量转基因样品进行检测。; [Objective]To establish a rapid DNA extraction method for the screening of transgenic rice.[Method]The kit method and fast methodof two DNA extraction methods were compared,and were proved by Ta NADP - ME1 and Ta NADP-ME2 gene transgenic rice respectively.[Result]PCR was done by using the DNA which extracted by the rapid method as template,about 1 700 bp Ta NADP-ME2 gene and about 1900bp Ta NADP-ME1 gene were obtained respectively,and the strips were clear and bright,comparing with the kit method showed no significantdifference.[Conclusion]The rapid DNA extraction method of this study is economic,simple and quick,suitable for the large transgenic samplesdetection.
Keyword转基因水稻 Dna快速提取 Pcr模板
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Document Type期刊论文
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GB/T 7714
苟小清,付振艳,王晓军. 一种用于转基因水稻快速检测的DNA提取方法[J]. 安徽农业科学,2012,40(35):17014-17015+17018.
APA 苟小清,付振艳,&王晓军.(2012).一种用于转基因水稻快速检测的DNA提取方法.安徽农业科学,40(35),17014-17015+17018.
MLA 苟小清,et al."一种用于转基因水稻快速检测的DNA提取方法".安徽农业科学 40.35(2012):17014-17015+17018.
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