XJIPC OpenIR  > 资源化学研究室
一种用于转基因水稻快速检测的DNA提取方法
苟小清; 付振艳; 王晓军
2012
Source Publication安徽农业科学
ISSN0517-6611
Volume40Issue:35Pages:17014-17015+17018
Abstract[目的]建立一种快速筛选转基因水稻的DNA提取方法。[方法]对试剂盒法和快速法2种DNA提取方法进行比较,并分别用转Ta NADP-ME1和Ta NADP-ME2基因的转基因水稻进行验证。[结果]用快速法提取得到的DNA进行PCR扩增,可分别得到约1 700 bp的Ta NADP-ME2基因和约1 900 bp的Ta NADP-ME1基因,且条带清晰明亮,与试剂盒法相比无显著差异。[结论]该研究中快速提取DNA的方法经济、简便、快捷,适合用于对大量转基因样品进行检测。; [Objective]To establish a rapid DNA extraction method for the screening of transgenic rice.[Method]The kit method and fast methodof two DNA extraction methods were compared,and were proved by Ta NADP - ME1 and Ta NADP-ME2 gene transgenic rice respectively.[Result]PCR was done by using the DNA which extracted by the rapid method as template,about 1 700 bp Ta NADP-ME2 gene and about 1900bp Ta NADP-ME1 gene were obtained respectively,and the strips were clear and bright,comparing with the kit method showed no significantdifference.[Conclusion]The rapid DNA extraction method of this study is economic,simple and quick,suitable for the large transgenic samplesdetection.
Keyword转基因水稻 Dna快速提取 Pcr模板
DOI10.13989/j.cnki.0517-6611.2012.35.117
Citation statistics
Document Type期刊论文
Identifierhttp://ir.xjipc.cas.cn/handle/365002/2440
Collection资源化学研究室
Affiliation中国科学院新疆理化技术研究所;中国科学院研究生院
Recommended Citation
GB/T 7714
苟小清,付振艳,王晓军. 一种用于转基因水稻快速检测的DNA提取方法[J]. 安徽农业科学,2012,40(35):17014-17015+17018.
APA 苟小清,付振艳,&王晓军.(2012).一种用于转基因水稻快速检测的DNA提取方法.安徽农业科学,40(35),17014-17015+17018.
MLA 苟小清,et al."一种用于转基因水稻快速检测的DNA提取方法".安徽农业科学 40.35(2012):17014-17015+17018.
Files in This Item:
File Name/Size DocType Version Access License
一种用于转基因水稻快速检测的DNA提取方(858KB)期刊论文作者接受稿开放获取CC BY-NC-SAView Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[苟小清]'s Articles
[付振艳]'s Articles
[王晓军]'s Articles
Baidu academic
Similar articles in Baidu academic
[苟小清]'s Articles
[付振艳]'s Articles
[王晓军]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[苟小清]'s Articles
[付振艳]'s Articles
[王晓军]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: 一种用于转基因水稻快速检测的DNA提取方法.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.