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林丽; 李进; 丁成丽
Source Publication食品科学

目的:建立利用高效液相色谱测定黑果枸杞果实中花色苷含量的方法。方法:采用Kromasil C18色谱柱(4.6mm×250mm,5μm)为固定相,0.2%甲酸溶液-乙腈(98:2)混合溶液为流动相梯度洗脱,用紫外检测器于520nm波长处检测,控制流量为1mL/min。结果:在43~935μg/mL范围内,相关系数为0.9994,RSD为2.73%,方法加标回收率为101.01%,相对标准偏差(n=6)为2.12%。结论:本方法简便,结果可靠,适用于黑果枸杞花色苷含量的测定。

Other Abstract

Objective: A HPLC method for the determination of anthocyanins in fruits of Lycium ruthenicum Murr. was developed. Methods: A Kromasil C18 (4.6 mm × 250 mm, 5 μm) column was used as the chromatographic column and a mixture of 0.2% HCOOH-H2O and acetonitrile (98:2) was used as the mobile phase at a flow rate of 1 mL/min for gradient elution. UV detection wavelength was set up at 520 nm. MAL standards were used for calibration. Results: An excellent linear relationship was achieved in the range of 43–935 μg/mL with correlation coefficient of 0.9994 and RSD of 2.73%. The recovery rate of in spiked samples was 101.01% with RSD (n = 6) of 2.12%. Conclusion: This developed method is simple, stable, fast, and reproducible and without the interference of impurities, so that it can be used for determining the content of anthocyanins in fruits of Lycium ruthenicum Murr..

Keyword高效液相色谱 花色苷 黑果枸杞
Indexed ByCSCD
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Cited Times:5[CSCD]   [CSCD Record]
Document Type期刊论文
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GB/T 7714
林丽,李进,丁成丽. 高效液相色谱法测定黑果枸杞果实中花色苷的含量[J]. 食品科学,2013,1.34(6):164-166.
APA 林丽,李进,&丁成丽.(2013).高效液相色谱法测定黑果枸杞果实中花色苷的含量.食品科学,1.34(6),164-166.
MLA 林丽,et al."高效液相色谱法测定黑果枸杞果实中花色苷的含量".食品科学 1.34.6(2013):164-166.
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