XJIPC OpenIR  > 省部共建新疆特有药用资源利用重点实验室
赵永昕; 热依木古丽·阿布都拉; 吴涛; 巴杭; 阿吉艾克拜尔·艾萨
Source Publication现代药物与临床


Other Abstract

Objective To establish a method for the isolation and preparation of isoquercetin by high speed counter-current chromatography (HSCCC). Methods The total flavonoids in Gossypii Flos extract was further separated by HSCCC with a two-phase solvent system composed of ethylacetate-water (1:1) stationary phase. Main engine speed was 850 r/min and the detection wavelength was 254 nm at a flow rate of 2.0 mL/min. The obtained fraction was identified by spectral analysis and the purity was analyzed by TLC and HPLC. Results Isoquercetin reference substance with the purity of 99% under this given situation was obtained. Conclusion The results indicate that HSCCC is a simple and rapid method for the separation and preparation of isoquercetin reference substance.

Keyword棉花花提取物 总黄酮 异槲皮素 高速逆流色谱
Document Type期刊论文
Recommended Citation
GB/T 7714
赵永昕,热依木古丽·阿布都拉,吴涛,等. 高速逆流色谱法从棉花花提取物中分离制备异槲皮素[J]. 现代药物与临床,2013,28(1):34-36.
APA 赵永昕,热依木古丽·阿布都拉,吴涛,巴杭,&阿吉艾克拜尔·艾萨.(2013).高速逆流色谱法从棉花花提取物中分离制备异槲皮素.现代药物与临床,28(1),34-36.
MLA 赵永昕,et al."高速逆流色谱法从棉花花提取物中分离制备异槲皮素".现代药物与临床 28.1(2013):34-36.
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