XJIPC OpenIR  > 省部共建新疆特有药用资源利用重点实验室
高速逆流色谱法从棉花花提取物中分离制备异槲皮素
赵永昕; 热依木古丽·阿布都拉; 吴涛; 巴杭; 阿吉艾克拜尔·艾萨
2013
Source Publication现代药物与临床
ISSN1674-5515
Volume28Issue:1Pages:34-36
Abstract

目的应用高速逆流色谱法分离制备异槲皮素。方法对棉花花提取物用高速逆流色谱法一步分离纯化,以醋酸乙酯-水(1:1)为两相溶剂体系,上相为固定相,下相为流动相,主机转速为800r/min,体积流量2.0mL/min,检测波长254nm,采用波谱法对所得化合物进行结构鉴定,薄层色谱、高效液相色谱法测定产品的纯度。结果在该条件下经一步分离可得到质量分数为99%的异槲皮素对照品。结论该方法操作简便,适合于从棉花花提取物中分离制备异槲皮素对照品。

Other Abstract

Objective To establish a method for the isolation and preparation of isoquercetin by high speed counter-current chromatography (HSCCC). Methods The total flavonoids in Gossypii Flos extract was further separated by HSCCC with a two-phase solvent system composed of ethylacetate-water (1:1) stationary phase. Main engine speed was 850 r/min and the detection wavelength was 254 nm at a flow rate of 2.0 mL/min. The obtained fraction was identified by spectral analysis and the purity was analyzed by TLC and HPLC. Results Isoquercetin reference substance with the purity of 99% under this given situation was obtained. Conclusion The results indicate that HSCCC is a simple and rapid method for the separation and preparation of isoquercetin reference substance.

Keyword棉花花提取物 总黄酮 异槲皮素 高速逆流色谱
Document Type期刊论文
Identifierhttp://ir.xjipc.cas.cn/handle/365002/2406
Collection省部共建新疆特有药用资源利用重点实验室
资源化学研究室
Affiliation中国科学院新疆理化技术研究所干旱区植物资源化学重点实验室
Recommended Citation
GB/T 7714
赵永昕,热依木古丽·阿布都拉,吴涛,等. 高速逆流色谱法从棉花花提取物中分离制备异槲皮素[J]. 现代药物与临床,2013,28(1):34-36.
APA 赵永昕,热依木古丽·阿布都拉,吴涛,巴杭,&阿吉艾克拜尔·艾萨.(2013).高速逆流色谱法从棉花花提取物中分离制备异槲皮素.现代药物与临床,28(1),34-36.
MLA 赵永昕,et al."高速逆流色谱法从棉花花提取物中分离制备异槲皮素".现代药物与临床 28.1(2013):34-36.
Files in This Item:
File Name/Size DocType Version Access License
高速逆流色谱法从棉花花提取物中分离制备异(724KB)期刊论文作者接受稿开放获取CC BY-NC-SAView Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[赵永昕]'s Articles
[热依木古丽·阿布都拉]'s Articles
[吴涛]'s Articles
Baidu academic
Similar articles in Baidu academic
[赵永昕]'s Articles
[热依木古丽·阿布都拉]'s Articles
[吴涛]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[赵永昕]'s Articles
[热依木古丽·阿布都拉]'s Articles
[吴涛]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: 高速逆流色谱法从棉花花提取物中分离制备异槲皮素.pdf
Format: Adobe PDF
This file does not support browsing at this time
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.