中国科学院新疆理化技术研究所机构知识库
Advanced  
XJIPC OpenIR  > 省部共建新疆特有药用资源利用重点实验室  > 期刊论文
题名: 高速逆流色谱法从棉花花提取物中分离制备异槲皮素
作者: 赵永昕; 热依木古丽·阿布都拉; 吴涛; 巴杭; 阿吉艾克拜尔·艾萨
关键词: 棉花花提取物 ; 总黄酮 ; 异槲皮素 ; 高速逆流色谱
刊名: 现代药物与临床
发表日期: 2013
卷: 28, 期:1, 页:34-36
资助者: 新疆维吾尔自治区高技术研究与发展项目(201010108)
摘要: 目的应用高速逆流色谱法分离制备异槲皮素。方法对棉花花提取物用高速逆流色谱法一步分离纯化,以醋酸乙酯-水(1:1)为两相溶剂体系,上相为固定相,下相为流动相,主机转速为800r/min,体积流量2.0mL/min,检测波长254nm,采用波谱法对所得化合物进行结构鉴定,薄层色谱、高效液相色谱法测定产品的纯度。结果在该条件下经一步分离可得到质量分数为99%的异槲皮素对照品。结论该方法操作简便,适合于从棉花花提取物中分离制备异槲皮素对照品。
英文摘要: Objective To establish a method for the isolation and preparation of isoquercetin by high speed counter-current chromatography (HSCCC). Methods The total flavonoids in Gossypii Flos extract was further separated by HSCCC with a two-phase solvent system composed of ethylacetate-water (1:1) stationary phase. Main engine speed was 850 r/min and the detection wavelength was 254 nm at a flow rate of 2.0 mL/min. The obtained fraction was identified by spectral analysis and the purity was analyzed by TLC and HPLC. Results Isoquercetin reference substance with the purity of 99% under this given situation was obtained. Conclusion The results indicate that HSCCC is a simple and rapid method for the separation and preparation of isoquercetin reference substance.
内容类型: 期刊论文
URI标识: http://ir.xjipc.cas.cn/handle/365002/2406
Appears in Collections:新疆维吾尔自治区植物资源化学重点实验室 _期刊论文

Files in This Item:
File Name/ File Size Content Type Version Access License
高速逆流色谱法从棉花花提取物中分离制备异槲皮素.pdf(724KB)期刊论文作者接受稿开放获取View 联系获取全文

作者单位: 中国科学院新疆理化技术研究所干旱区植物资源化学重点实验室

Recommended Citation:
赵永昕,热依木古丽·阿布都拉,吴涛,等. 高速逆流色谱法从棉花花提取物中分离制备异槲皮素[J]. 现代药物与临床,2013,28(1):34-36.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[赵永昕]'s Articles
[热依木古丽·阿布都拉]'s Articles
[吴涛]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[赵永昕]‘s Articles
[热依木古丽·阿布都拉]‘s Articles
[吴涛]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
文件名: 高速逆流色谱法从棉花花提取物中分离制备异槲皮素.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Powered by CSpace