用紫外可见分光光度法测定共轭亚油酸的含量
Alternative Titlequantitation of coniugated linoleic acid by uv spectrophotometry
张亚刚; 樊莉; 马莉; 文彬; 吾满江·艾力
2002
Source Publication中国科学院研究生院学报
ISSN1002-1175
Volume19Issue:4Pages:436-442
Abstract

采用UV 91 0 0型紫外可见分光光度计,测定了共轭亚油酸、共轭亚油酸甲酯、共轭亚油酸三甘酯在不同浓度时在 2 0 0~ 3 0 0nm的吸光度,绘制出样品在不同浓度时的吸光度曲线,找出适宜的浓度范围;用MicrocalSoftwareOriginVersion 4.0做回归分析,由线性回归给出样品浓度和吸光度的线性关系;利用PEAutosystemXL TurboMass测得样品中共轭亚油酸乙酯的准确浓度,结合回归系数给出了测定样品中共轭亚油酸含量的经验公式.

Other Abstract

Through absorbance measurement of conjugated linoleic acid, methyl ester of conjugated lin-oleic acid and conjugated linoleic acid triglycide from 210-~300 nm by UV-9100 spectrophotometer, ab-sorbancy curve of measured samples at different concentration is plotted. In the appropriate concentra-tion range of samples, linear relationship of absorbancy and samples' concentration is presented by line-ar regression with Microcal Software Origin Version 4. 0 system. The accurate concentration of conj u-gated linoleic acid is obtained with PE Autosystem XL-TruboMass. Combined with regression constant data , emprical formula for the quantitation of conjugated linoleic acid by UV spectrophotometer is giv-en.

KeywordUltraviolet-visible Spectrophotometry Conjugated Linoleic Acid Octadecadienoic Acid
Subject AreaEngineering (Provided By Thomson Reuters)
Indexed ByCSCD
CSCD IDCSCD:1366507
Citation statistics
Cited Times:11[CSCD]   [CSCD Record]
Document Type期刊论文
Identifierhttp://ir.xjipc.cas.cn/handle/365002/2271
Collection新疆维吾尔自治区精细化工工程技术研究中心
资源化学研究室
Affiliation中国科学院新疆理化技术研究所; 中国科学院研究生院
Recommended Citation
GB/T 7714
张亚刚,樊莉,马莉,等. 用紫外可见分光光度法测定共轭亚油酸的含量[J]. 中国科学院研究生院学报,2002,19(4):436-442.
APA 张亚刚,樊莉,马莉,文彬,&吾满江·艾力.(2002).用紫外可见分光光度法测定共轭亚油酸的含量.中国科学院研究生院学报,19(4),436-442.
MLA 张亚刚,et al."用紫外可见分光光度法测定共轭亚油酸的含量".中国科学院研究生院学报 19.4(2002):436-442.
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