Through absorbance measurement of conjugated linoleic acid, methyl ester of conjugated lin-oleic acid and conjugated linoleic acid triglycide from 210-~300 nm by UV-9100 spectrophotometer, ab-sorbancy curve of measured samples at different concentration is plotted. In the appropriate concentra-tion range of samples, linear relationship of absorbancy and samples' concentration is presented by line-ar regression with Microcal Software Origin Version 4. 0 system. The accurate concentration of conj u-gated linoleic acid is obtained with PE Autosystem XL-TruboMass. Combined with regression constant data , emprical formula for the quantitation of conjugated linoleic acid by UV spectrophotometer is giv-en.