中国科学院新疆理化技术研究所机构知识库
Advanced  
XJIPC OpenIR  > 资源化学研究室  > 期刊论文
题名: 用酵母双杂交系统研究载脂蛋白AI(apoAI)和清道夫受体BI(SR-BI)间的相互作用
其他题名: study on interaction between apolipoprotein ai and scavenger receptors class b type i by yeast two-hybrid
作者: 信学雷; 陈志慧; 李维琪; 刘云英; 麦迪娜; 张云峰
关键词: 酵母双杂交 载脂蛋白AI 清道夫受体BI 相互作用
刊名: 中国生物工程杂志
发表日期: 2005
卷: 25, 期:5, 页:80-84
资助者: 中国科学院“西部之光”资助项目
摘要: 目的:利用酵母双杂交系统验证在胆固醇逆转运过程中起关键作用的大鼠载脂蛋白AI(apoAI)和清道夫受体BI- (SR -BI)间存在着相互作用,为初步筛选具有降脂活性组分提供1对新的靶点。方法:首先分别克隆了Wistar大鼠的apoAI和SR -BI基因的cDNA ,并构建了酵母表达载体,利用共转化技术观察到apoAI和SR -BI间存在着相互作用,并在酵母交配实验中证实了这个结果。结果:经共转化后的实验组与阳性对照组可在SD/ -Leu/- Trp/- His/- Ade/X -α- Gal平板上生长且菌斑呈蓝色,经测定α、β半乳糖苷酶活力可知酶活分别为8~1 2U和1 0~40U。酵母交配后的二倍体实验组、阳性对照组可在SD/- Leu/- Trp/ -His/- Ade/X -α- Gal平板上生长且菌斑呈蓝色。结论:apoAI和SR BI间的确存在相互作用。
英文摘要: Objective: To verify the interaction between apolipoprotein AI and scavenger receptors class B type I (SR-BI) , a key process of reverse cholesterol transport, by yeast two-hybrid method and to provide a new target for selecting active substance for reducing lipid in plasma. Method: Apolipoprotein AI and SR-BI gene cDNA from Wistar rat were cloned, and DNA/apoAI and AD/SR-BI were constructed, then co-transformed into yeast strain AH109. Transformants were selected on medium of SD/-Leu/-Trp/-His/-Ade/X-a-Gal and the putative positive clones were identified by yeast mating. Result: Transformants can grow on medium of SD/-Leu/-Trp/-His/-Ade/X-a-Gal and the colonies are blue, an (3- galactosidase assays show that the activity of ou [3-galactosidase are 8 ~ 12U and 10 ~ 40U respectively. After yeast mating, blue colonies can still be seen on medium of SD/-Leu/-Trp/-His/-Ade/X-a-Gal. Conclusion: It is true that there is interaction between apolipoprotein AI and SR-BI.
内容类型: 期刊论文
URI标识: http://ir.xjipc.cas.cn/handle/365002/2234
Appears in Collections:资源化学研究室_期刊论文

Files in This Item:
File Name/ File Size Content Type Version Access License
用酵母双杂交系统研究载脂蛋白AI(apoAI)和清道夫受体BI(SR-BI)间的相互作用.pdf(220KB)期刊论文作者接受稿开放获取View 联系获取全文

作者单位: 中国科学院新疆理化技术研究所

Recommended Citation:
信学雷,陈志慧,李维琪,等. 用酵母双杂交系统研究载脂蛋白AI(apoAI)和清道夫受体BI(SR-BI)间的相互作用[J]. 中国生物工程杂志,2005,25(5):80-84.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[信学雷]'s Articles
[陈志慧]'s Articles
[李维琪]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[信学雷]‘s Articles
[陈志慧]‘s Articles
[李维琪]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
文件名: 用酵母双杂交系统研究载脂蛋白AI(apoAI)和清道夫受体BI(SR-BI)间的相互作用.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Powered by CSpace