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Preparative isolation and purification of rupestonic acid from the Chinese medicinal plant Artemisia rupestris L. by high-speed counter-current chromatography
Ma Yanming; Aisha Haji Akber; Liao Lixin; Aibai Sirafil; Zhang Tianyou; Ito Yoichiro
2005
发表期刊Journal of Chromatography A
ISSN219673
卷号1076期号:40910页码:198-201
摘要Rupestonic acid was purified for the first time by high-speed counter-current chromatography from a dichloromethane extract of the traditional Chinese medicinal plant Artemisia rupestris L. The separation was performed in two steps with a two-phase solvent system composed of n-hexane-ethyl acetate-methanol-water (6:4:3.5:6.5, v/v) with 0.5% acetic acid in stationary-phase. From 200 mg of the crude extract, 27.9 mg of rupestonic acid was obtained at over 98% purity as determined by HPLC analysis, and its chemical structure was confirmed by MS, 1H and 13C nuclear magnetic resonance.
关键词Artemisia Rupestris L. Counter-current Chromatography Rupestonic Acid
DOI10.1016/j.chroma.2005.04.040
收录类别SCI
WOS记录号WOS:000229551000027
引用统计
被引频次:22[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.xjipc.cas.cn/handle/365002/2216
专题资源化学研究室
作者单位NHLBI, Ctr Biochem & Biophys, NIH, Bethesda, MD 20892 USA;CAS, Xinjiang Tech Ins Phys & Chem, Urumqi 830011, Peoples R China;Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China;Inst Xinjiang Uyghur Med, Urumqi 83001, Peoples R China;Beijing Inst New Technol Appl, Beijing 100035, Peoples R China
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Ma Yanming,Aisha Haji Akber,Liao Lixin,et al. Preparative isolation and purification of rupestonic acid from the Chinese medicinal plant Artemisia rupestris L. by high-speed counter-current chromatography[J]. Journal of Chromatography A,2005,1076(40910):198-201.
APA Ma Yanming,Aisha Haji Akber,Liao Lixin,Aibai Sirafil,Zhang Tianyou,&Ito Yoichiro.(2005).Preparative isolation and purification of rupestonic acid from the Chinese medicinal plant Artemisia rupestris L. by high-speed counter-current chromatography.Journal of Chromatography A,1076(40910),198-201.
MLA Ma Yanming,et al."Preparative isolation and purification of rupestonic acid from the Chinese medicinal plant Artemisia rupestris L. by high-speed counter-current chromatography".Journal of Chromatography A 1076.40910(2005):198-201.
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