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学科主题: Cell Biology (provided by Thomson Reuters)
题名: 酵母双杂交衍生系统
其他题名: yeast two-hybrid derived system
作者: 马海蓉; 李维琪
关键词: Yeast two-hybrid system ; Protein-protein interactions ; Derived yeast-hybrid system
刊名: 中国生物工程杂志
发表日期: 2003
DOI: 10.13523/j.cb.20030208
卷: 23, 期:2, 页:37-41
摘要: 酵母双杂交系统是在酵母体内分析蛋白质 蛋白质相互作用的基因系统 ,由Fields等人于1 989年首次建立并得到广泛地应用。十余年来 ,随着酵母双杂交迅速推广 ,不断涌现出一些衍生系统 ,其中包括酵母双杂交的二元诱饵系统 ,逆向双杂交系统 ,非转录读出特点的双杂交系统 (如Sos蛋白招募系统、PI3K介导的靶蛋白识别系统和断裂 泛素为基础的双杂交系统 )以及转录激活因子与其相关蛋白之间的相互作用的双杂交系统 (如以polⅢ为基础的杂交系统和RTA系统 )等。它们的建立在很大程度上克服了传统酵母双杂交系统的局限性 ,扩大了被研究的蛋白质的范围 ,提高了系统的灵敏度。
英文摘要: Yeast two-hybrid system is a genetic means of analyzing protein-protein interaction in vivo . It was first described by Fields in 1989 and has been widely used since then, but it itself had a few limits. In recent years, some derived yeast two-hybrid systems have been established,including yeast two-hybrid dual bait system,reverse two-hybrid system, membrane-associated two-hybrid system ( SRS, TOPIS and split-ubiquitin base two-hybrid system) and transcriptional factors-associated two-hybrid system (RTA system and Pol III based two-hybrid system) .which promise to overcome some limits and improved the sensitivities of the original yeast two-hybrid system. In this review, the principles and applications of the derived yeast two-hybrid systems are introduced .
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内容类型: 期刊论文
URI标识: http://ir.xjipc.cas.cn/handle/365002/2175
Appears in Collections:资源化学研究室_期刊论文

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作者单位: 中国科学院新疆理化技术研究所;中国科学院研究生院

Recommended Citation:
马海蓉,李维琪. 酵母双杂交衍生系统[J]. 中国生物工程杂志,2003,23(2):37-41.
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