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Alternative Titlethe optimization of cell suspensiong culture protocol of lavandula angustifolia, cv. munstead
许耀祖; 王晓军; 赵民安; 韦彦余; 赵海清; 林侃
Source Publication细胞生物学杂志


Other Abstract

By the orthogonalty design, the factors influencing suspension cells growth of L.angustifolia were studied, and best culture conditions which were B5 media supplemented with 40 g/L sucrose, 0.025 mg/L 2,4-D, 0.5 mg/L 6-BA and the rotating speed of 120 r/min were sifted. After 15 days of culture, the density of surviving cells could reach 4.4x105 cell/ml, and differentiation rate of suspension cells could surpass 90% after 3 months of suspension culture. The cause of effects of sucrose concentration on the density of surviving cells was discussed through the analysis of the consumption rule of surplus sucrose

Keyword薰衣草 细胞悬浮培养 正交设计
Subject AreaCell Biology (Provided By Thomson Reuters)
Indexed ByCSCD
Citation statistics
Cited Times:6[CSCD]   [CSCD Record]
Document Type期刊论文
Recommended Citation
GB/T 7714
许耀祖,王晓军,赵民安,等. 薰衣草细胞悬浮培养体系的优化[J]. 细胞生物学杂志,2005,27(6):701-704.
APA 许耀祖,王晓军,赵民安,韦彦余,赵海清,&林侃.(2005).薰衣草细胞悬浮培养体系的优化.细胞生物学杂志,27(6),701-704.
MLA 许耀祖,et al."薰衣草细胞悬浮培养体系的优化".细胞生物学杂志 27.6(2005):701-704.
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