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Alternative Titlepreparation of rupestonic acid reference substance by preparative hplc
马雁鸣; 赵芸; 吴韬; 斯拉甫; 廖立新; 阿吉艾克拜尔.艾萨
Source Publication中草药


Other Abstract

Objective To establish a preparative method for rupestonic acid reference substance from Artemisia rupestris by HPLC. Methods After raw material being extracted with 95% ethanol, the ethanol extracts were evaporated to dryness under reduced pressure. Then the dry powder was extracted by ethyl acetate, the ethyl acetate extract was fractionated by column chromatography on silica gel (100200 mesh.) using petroleumbenzine-ethyl acetate (3 ! 1) as the eluent. The collected fraction was purified by preparative HPLC Results The purity of the product quantitated by normality was over 98%. t-onclusion The developed method is simple and rapid and the product of rupestonic acid can be used as reference substance.

Keyword新疆一枝蒿 一枝蒿酮酸 对照品 制备高效液相色谱
Subject AreaGeneral & Internal Medicine (Provided By Thomson Reuters)
Indexed ByCSCD
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Document Type期刊论文
Corresponding Author阿吉艾克拜尔.艾萨
Recommended Citation
GB/T 7714
马雁鸣,赵芸,吴韬,等. 高效液相制备色谱制备一枝蒿酮酸对照品[J]. 中草药,2005,36(7):47-49.
APA 马雁鸣,赵芸,吴韬,斯拉甫,廖立新,&阿吉艾克拜尔.艾萨.(2005).高效液相制备色谱制备一枝蒿酮酸对照品.中草药,36(7),47-49.
MLA 马雁鸣,et al."高效液相制备色谱制备一枝蒿酮酸对照品".中草药 36.7(2005):47-49.
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