Linoleid acids （LA） were sampled in glass bottles added with VE and conjugated linoleic acid （CLA） respectively, and were irradiated with ^60Co at different dose lever. Peroxide value of samples was determined every several days. The results showed that the VE and CLA had great effect on antioxidation of samples. CLA is better than VE in antioxidation. Antioxidation mechanism of VE and CLA on irradiated LA was discussed.