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人apoA-I分泌型表达调控细胞模型构建
Alternative Titlethe cell pattern construction of human apoa-i gene regulation and secretory expression
孟葆林; 李维琪; 毛建平; 曹旭
2005
Source Publication中国生物工程杂志
ISSN1671-8135
Volume25Issue:8Pages:39-44
Abstract

分别从pMD18-T质粒和人基因组DNA扩增出人apoA-I CDS区序列和apoA-I启动子(702bp片段),与pEGFP-N1重组,构成受apoA-I启动子调控的pEGFP-N1质粒和融合蛋白表达质粒,分别转染人肝癌HepG2细胞,以绿色荧光为标志筛选稳定转染系列克隆。用RT-PCR、荧光显微镜、免疫荧光术等鉴定其中一个克隆融合蛋白的表达;分别以胰岛素和葡萄糖刺激物鉴定该克隆的外源apoA-I启动子调控。结果表明:人apoA-I分泌型表达调控肝细胞模型初步建成。

Keyword载脂蛋白a-i Apoa-i启动子 增强型绿色荧光蛋白 人肝癌细胞hepg2 稳定转染克隆
DOI10.13523/j.cb.20050808
Indexed ByCSCD
CSCD IDCSCD:2172306
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Document Type期刊论文
Identifierhttp://ir.xjipc.cas.cn/handle/365002/2158
Collection资源化学研究室
Affiliation中国科学院新疆理化技术研究所;中国科学院研究生院
Recommended Citation
GB/T 7714
孟葆林,李维琪,毛建平,等. 人apoA-I分泌型表达调控细胞模型构建[J]. 中国生物工程杂志,2005,25(8):39-44.
APA 孟葆林,李维琪,毛建平,&曹旭.(2005).人apoA-I分泌型表达调控细胞模型构建.中国生物工程杂志,25(8),39-44.
MLA 孟葆林,et al."人apoA-I分泌型表达调控细胞模型构建".中国生物工程杂志 25.8(2005):39-44.
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