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题名: 大鼠SR-BI胞外域与ApoA-I蛋白相互作用的确认
其他题名: verification the interaction of apolipoprotein ai and the extracellular domain of scavenger receptors class b type i
作者: 李兰松; 李维琪; 信学雷; 曹旭
关键词: 载脂蛋白AI(ApoA-I) ; B族I型清道夫受体(SR-BI) ; SR-BI胞外域 ; 基因克隆 ; 酵母双杂交
刊名: 微生物学通报
发表日期: 2006
DOI: 10.13344/j.microbiol.china.2006.02.016
卷: 33, 期:2, 页:68-73
资助者: 中国科学院“百人计划”项目资助;2001年“西部之光”项目资助
摘要: 先以含全长SR-BI cDNA序列的重组质粒pMD18-T-rS为摸板进行PCR反应扩增SRBI得到胞外域cDNA片段,经测序证明正确后,定向克隆到酵母双杂交表达载体,然后与pGBKT7-ApoA-I质粒共转化酵母细胞,通过报告基因及酵母交配试验确认了SR-BI的胞外域部分和ApoA-I之间的确存,观察到ApoA-I与SR-BI胞外域间的相互作用力比与全长的SR-BI间的相互作用力提高了10%.
英文摘要: In this paper, the DNA fragment rSE was amplified by PCR using the recombinant plasmids pMD18- T-rS (containing the hole lines of cDNA of SR-BI) as template. Then the PCR product was directionally cloned into the yeast two hybrid express vector pGADT7 after it was confirmed with sequencing. The recombined vector (named pGADTT-rSE) and pGBKTT-ApoA-Ⅰ vector was cotransformed in yeast strain AH109. We identified the interaction of APOA-Ⅰ and rSE by using report genes and yeast mating, At last, we obsevered that the interaction of APOA-Ⅰ and rSE is about 10% more than that of ApoA-Ⅰ and SR-BI of rat.
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内容类型: 期刊论文
URI标识: http://ir.xjipc.cas.cn/handle/365002/2110
Appears in Collections:资源化学研究室_期刊论文

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作者单位: 中国科学院新疆理化技术研究所;中国科学院研究生院

Recommended Citation:
李兰松,李维琪,信学雷,等. 大鼠SR-BI胞外域与ApoA-I蛋白相互作用的确认[J]. 微生物学通报,2006,33(2):68-73.
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文件名: 大鼠SR-BI胞外域与ApoA-I蛋白相互作用的确认.pdf
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