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大鼠SR-BI胞外域与ApoA-I蛋白相互作用的确认
Alternative Titleverification the interaction of apolipoprotein ai and the extracellular domain of scavenger receptors class b type i
李兰松; 李维琪; 信学雷; 曹旭
2006
Source Publication微生物学通报
ISSN0253-2654
Volume33Issue:2Pages:68-73
Abstract

先以含全长SR-BI cDNA序列的重组质粒pMD18-T-rS为摸板进行PCR反应扩增SRBI得到胞外域cDNA片段,经测序证明正确后,定向克隆到酵母双杂交表达载体,然后与pGBKT7-ApoA-I质粒共转化酵母细胞,通过报告基因及酵母交配试验确认了SR-BI的胞外域部分和ApoA-I之间的确存,观察到ApoA-I与SR-BI胞外域间的相互作用力比与全长的SR-BI间的相互作用力提高了10%.

Other Abstract

In this paper, the DNA fragment rSE was amplified by PCR using the recombinant plasmids pMD18- T-rS (containing the hole lines of cDNA of SR-BI) as template. Then the PCR product was directionally cloned into the yeast two hybrid express vector pGADT7 after it was confirmed with sequencing. The recombined vector (named pGADTT-rSE) and pGBKTT-ApoA-Ⅰ vector was cotransformed in yeast strain AH109. We identified the interaction of APOA-Ⅰ and rSE by using report genes and yeast mating, At last, we obsevered that the interaction of APOA-Ⅰ and rSE is about 10% more than that of ApoA-Ⅰ and SR-BI of rat.

Keyword载脂蛋白ai(Apoa-i) B族i型清道夫受体(Sr-bi) Sr-bi胞外域 基因克隆 酵母双杂交
DOI10.13344/j.microbiol.china.2006.02.016
Indexed ByCSCD
CSCD IDCSCD:2334669
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Document Type期刊论文
Identifierhttp://ir.xjipc.cas.cn/handle/365002/2110
Collection资源化学研究室
Affiliation中国科学院新疆理化技术研究所;中国科学院研究生院
Recommended Citation
GB/T 7714
李兰松,李维琪,信学雷,等. 大鼠SR-BI胞外域与ApoA-I蛋白相互作用的确认[J]. 微生物学通报,2006,33(2):68-73.
APA 李兰松,李维琪,信学雷,&曹旭.(2006).大鼠SR-BI胞外域与ApoA-I蛋白相互作用的确认.微生物学通报,33(2),68-73.
MLA 李兰松,et al."大鼠SR-BI胞外域与ApoA-I蛋白相互作用的确认".微生物学通报 33.2(2006):68-73.
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