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题名: Preparation and characterization of mono-PEGylated consensus interferon by a novel polyethylene glycol derivative
作者: Yun Qiang; Xing Wenchao; Ma Guanghui; Su Zhiguo
关键词: consensus interferon ; chromatography ; PEGylation ; MALDI-TOF
刊名: Journal of Chemical Technology and Biotechnology
发表日期: 2006
DOI: 10.1002/jctb.1470
卷: 81, 期:5, 页:776-781
收录类别: SCI
英文摘要: Traditional PEGylation strategy of peptide and protein usually results in heterogeneous products wide molecular weight distribution. Mono-PEGylated consensus interferon (C-IFN) with homogeneous molecular weight was prepared by a novel methoxypolyethylene glycol (mPEG) derivative. 2-Fluoro-1-methylpyridinium toluene-4-sulfonate (FMP) was applied to activate mPEG and a novel functionalized mPEG derivative (FMP-mPEG) was obtained. The reactivity of FMP-mPEG toward amino groups of C-IFN was evaluated, and the heterogeneity of PEGylated protein was identified by high-performance size-exclusion chromatography (HPSEC) and matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS). Finally, the PEGylated C-IFN was separated from intact protein by cation-exchange chromatography. The results indicated that PEGylation process was dependent on pH dramatically and the modification degree was increased with the increasing molar excess of FMP-mPEG. Under optimized condition, mono-PEGylated C-IFN with molecule weight of 24.64 kDa was achieved. Further investigation of in vitro biological activities demonstrated that about 88% of antiviral activity was maintained by the mono-PEGylated C-IFN.
WOS记录号: WOS:000237364500008
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内容类型: 期刊论文
URI标识: http://ir.xjipc.cas.cn/handle/365002/2034
Appears in Collections:资源化学研究室_期刊论文

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作者单位: Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100080, Peoples R China;Chinese Acad Sci, Xinjiang Tech Inst Phys & Chem, Urumqi 830011, Peoples R China

Recommended Citation:
Yun Qiang,Xing Wenchao,Ma Guanghui,et al. Preparation and characterization of mono-PEGylated consensus interferon by a novel polyethylene glycol derivative[J]. Journal of Chemical Technology and Biotechnology,2006,81(5):776-781.
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