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Preparation and characterization of mono-PEGylated consensus interferon by a novel polyethylene glycol derivative
Yun Qiang; Xing Wenchao; Ma Guanghui; Su Zhiguo
2006
Source PublicationJournal of Chemical Technology and Biotechnology
ISSN2682575
Volume81Issue:5Pages:776-781
AbstractTraditional PEGylation strategy of peptide and protein usually results in heterogeneous products wide molecular weight distribution. Mono-PEGylated consensus interferon (C-IFN) with homogeneous molecular weight was prepared by a novel methoxypolyethylene glycol (mPEG) derivative. 2-Fluoro-1-methylpyridinium toluene-4-sulfonate (FMP) was applied to activate mPEG and a novel functionalized mPEG derivative (FMP-mPEG) was obtained. The reactivity of FMP-mPEG toward amino groups of C-IFN was evaluated, and the heterogeneity of PEGylated protein was identified by high-performance size-exclusion chromatography (HPSEC) and matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS). Finally, the PEGylated C-IFN was separated from intact protein by cation-exchange chromatography. The results indicated that PEGylation process was dependent on pH dramatically and the modification degree was increased with the increasing molar excess of FMP-mPEG. Under optimized condition, mono-PEGylated C-IFN with molecule weight of 24.64 kDa was achieved. Further investigation of in vitro biological activities demonstrated that about 88% of antiviral activity was maintained by the mono-PEGylated C-IFN.
KeywordConsensus Interferon Chromatography Pegylation Maldi-tof
DOI10.1002/jctb.1470
Indexed BySCI
WOS IDWOS:000237364500008
Citation statistics
Cited Times:5[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.xjipc.cas.cn/handle/365002/2034
Collection资源化学研究室
AffiliationChinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100080, Peoples R China;Chinese Acad Sci, Xinjiang Tech Inst Phys & Chem, Urumqi 830011, Peoples R China
Recommended Citation
GB/T 7714
Yun Qiang,Xing Wenchao,Ma Guanghui,et al. Preparation and characterization of mono-PEGylated consensus interferon by a novel polyethylene glycol derivative[J]. Journal of Chemical Technology and Biotechnology,2006,81(5):776-781.
APA Yun Qiang,Xing Wenchao,Ma Guanghui,&Su Zhiguo.(2006).Preparation and characterization of mono-PEGylated consensus interferon by a novel polyethylene glycol derivative.Journal of Chemical Technology and Biotechnology,81(5),776-781.
MLA Yun Qiang,et al."Preparation and characterization of mono-PEGylated consensus interferon by a novel polyethylene glycol derivative".Journal of Chemical Technology and Biotechnology 81.5(2006):776-781.
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