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新疆雪莲体细胞胚胎发生
Alternative Titlesomatic embryogenesis of saussurea involucrata kar.et kir.
贾莉芳; 王晓军; 赵民安
2008
Source Publication植物学通报
ISSN1003-2266
Volume25Issue:1Pages:85-88
Abstract

通过体细胞胚胎发生途径实现了新疆雪莲(Saussurea involucrata Kar.etKir.)的植株再生。选用新疆雪莲子叶为外植体,接种于MS+0.5mg.L-12,4-D+0.05-1mg.L-1BA的固体培养基上,进行愈伤组织的诱导。从第1次继代培养的愈伤组织中挑选出黄绿色、颗粒状、质地致密的胚性愈伤组织,转移到含0.05-0.1mg.L-12,4-D的MS液体培养基中进行悬浮培养,20天后可分化产生大量球形胚。继代过程中相继加入PEG和GA3,可以促进体细胞胚的分化和生长。体细胞胚在含有5mg.L-1GA3的MS固体培养基上,可发育成完整的植株。

Keyword植株再生 新疆雪莲 体细胞胚胎发生 组织培养
Indexed ByCSCD
CSCD IDCSCD:3209345
Citation statistics
Cited Times:4[CSCD]   [CSCD Record]
Document Type期刊论文
Identifierhttp://ir.xjipc.cas.cn/handle/365002/2023
Collection资源化学研究室
Affiliation中国科学院新疆理化技术研究所; 中国科学院研究生院
Recommended Citation
GB/T 7714
贾莉芳,王晓军,赵民安. 新疆雪莲体细胞胚胎发生[J]. 植物学通报,2008,25(1):85-88.
APA 贾莉芳,王晓军,&赵民安.(2008).新疆雪莲体细胞胚胎发生.植物学通报,25(1),85-88.
MLA 贾莉芳,et al."新疆雪莲体细胞胚胎发生".植物学通报 25.1(2008):85-88.
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