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新疆天山雪莲体胚诱导与分化研究
林侃; 王晓军; 赵民安; 刘敏; 努尔波拉提
2006
Source Publication西北植物学报
ISSN1000-4025
Volume26Issue:7Pages:1351-1354
Abstract

以新疆天山雪莲的叶片为外植体,分别用不同配方培养基诱导愈伤组织,后进行体胚诱导和分化培养形成再生雪莲植株.结果表明,诱导愈伤组织的最适培养基为MS+2,4-D 0.5 mg/L+BA 1.5 mg/L,诱导率可达到100%;愈伤组织转移至MS+2,4-D 0.5 mg/L+BA 1.5 mg/L培养基进行继代培养,增殖后的愈伤组织转移到MS+2,4-D 0.2 mg/L的液体培养基后成功诱导出雪莲体胚,出胚率达40%;将体胚接至MS+ABA 0.5 mg/L培养基后,结果分化生长出大量的再生雪莲幼苗.

Keyword新疆天山雪莲 愈伤 体胚
Indexed ByCSCD
CSCD IDCSCD:2510967
Citation statistics
Cited Times:5[CSCD]   [CSCD Record]
Document Type期刊论文
Identifierhttp://ir.xjipc.cas.cn/handle/365002/1943
Collection资源化学研究室
Affiliation中国科学院新疆理化技术研究所;中国科学院研究生院
Recommended Citation
GB/T 7714
林侃,王晓军,赵民安,等. 新疆天山雪莲体胚诱导与分化研究[J]. 西北植物学报,2006,26(7):1351-1354.
APA 林侃,王晓军,赵民安,刘敏,&努尔波拉提.(2006).新疆天山雪莲体胚诱导与分化研究.西北植物学报,26(7),1351-1354.
MLA 林侃,et al."新疆天山雪莲体胚诱导与分化研究".西北植物学报 26.7(2006):1351-1354.
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