XJIPC OpenIR  > 资源化学研究室
Preparative isolation of guaipyridine sesquiterpene alkaloid from Artemisia rupestris L. flowers using high-speed counter-current chromatography
Su Zhen; Wu Hankui; Aisa Haji Akber; Slukhan Usmanova; Aripova Salimakhon; Aisa, HA
2008
Source PublicationJournal of Separation Science
ISSN1615-9306
Volume31Issue:12Pages:2161-2166
Abstract

Although the medicinal plant Artemisia rupestris L. has been widely researched for several decades, its alkaloids have never been isolated before. To our surprise, the alkaloids in the plant were not detected in the stems but detected in the flowers. Herein, a novel and strange guaipyridine sesquiterpene alkaloid with a carboxyl group named rupestine was purified successfully from the total alkaloids extracted from the flowers by high-speed counter-current chromatography (HSCCC). The two-phase solvent system used was composed of ethyl acetate-methanol-water (8:1:7, v/v/v). Fifty six milligrams of rupestine was obtained at over 97% purity and 95% recovery from 200 mg of the total alkaloids in one-step separation. Its structure was elucidated by spectroscopic methods including high resolution ESI-MS, 1H NMR, 13C NMR, Heteronuclear Multiple Bond Correlation (HMBC), Heteronuclear Single Quantum Coherence (HSQC), and Nuclear Overhauser Enhancement Spectroscopy (NOESY). © 2008 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

KeywordArtemisia Rupestris L. High-speed Counter-current Chromatography Rupestine 2-(2' 5'alpha-dimethyl-6' 7' 8'alpha 9'-tetrahydro-5'beta H-cyclohepta[Blpyridine-8'beta-yl)-acrylic Acid
DOI10.1002/jssc.200700683
Indexed BySCI
WOS IDWOS:000257768200004
Citation statistics
Cited Times:17[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.xjipc.cas.cn/handle/365002/1934
Collection资源化学研究室
Corresponding AuthorAisa, HA
Affiliation1.Chinese Acad Sci, Xinjiang Tech Inst Phys & Chem, Xinjiang Key Lab Plant Resources & Nat Prod Chem, Urumqi 830011, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China
3.Acad Sci Uzbek, Yunusov Inst Chem Plang Substances, Tashkent, Uzbekistan
Recommended Citation
GB/T 7714
Su Zhen,Wu Hankui,Aisa Haji Akber,et al. Preparative isolation of guaipyridine sesquiterpene alkaloid from Artemisia rupestris L. flowers using high-speed counter-current chromatography[J]. Journal of Separation Science,2008,31(12):2161-2166.
APA Su Zhen,Wu Hankui,Aisa Haji Akber,Slukhan Usmanova,Aripova Salimakhon,&Aisa, HA.(2008).Preparative isolation of guaipyridine sesquiterpene alkaloid from Artemisia rupestris L. flowers using high-speed counter-current chromatography.Journal of Separation Science,31(12),2161-2166.
MLA Su Zhen,et al."Preparative isolation of guaipyridine sesquiterpene alkaloid from Artemisia rupestris L. flowers using high-speed counter-current chromatography".Journal of Separation Science 31.12(2008):2161-2166.
Files in This Item:
File Name/Size DocType Version Access License
preparative isolatio(513KB)期刊论文作者接受稿开放获取CC BY-NC-SAView Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Su Zhen]'s Articles
[Wu Hankui]'s Articles
[Aisa Haji Akber]'s Articles
Baidu academic
Similar articles in Baidu academic
[Su Zhen]'s Articles
[Wu Hankui]'s Articles
[Aisa Haji Akber]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Su Zhen]'s Articles
[Wu Hankui]'s Articles
[Aisa Haji Akber]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: preparative isolation of guaipyridine sesquiterpene alkaloid from artemisia rupestris l. flowers using high-speed counter-current chromatography.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.