题名: Preparative isolation of guaipyridine sesquiterpene alkaloid from Artemisia rupestris L. flowers using high-speed counter-current chromatography
作者: Su Zhen; Wu Hankui; Aisa Haji Akber; Slukhan Usmanova; Aripova Salimakhon
通讯作者: Aisa, HA
关键词: Artemisia rupestris L.
; high-speed counter-current chromatography
; 2-(2',5'alpha-dimethyl-6',7',8'alpha,9'-tetrahydro-5'beta H-cyclohepta[blpyridine-8'beta-yl)-acrylic acid
刊名: Journal of Separation Science
卷: 31, 12, 2161-2166
摘要: Although the medicinal plant Artemisia rupestris L. has been widely researched for several decades, its alkaloids have never been isolated before. To our surprise, the alkaloids in the plant were not detected in the stems but detected in the flowers. Herein, a novel and strange guaipyridine sesquiterpene alkaloid with a carboxyl group named rupestine was purified successfully from the total alkaloids extracted from the flowers by high-speed counter-current chromatography (HSCCC). The two-phase solvent system used was composed of ethyl acetate-methanol-water (8:1:7, v/v/v). Fifty six milligrams of rupestine was obtained at over 97% purity and 95% recovery from 200 mg of the total alkaloids in one-step separation. Its structure was elucidated by spectroscopic methods including high resolution ESI-MS, 1H NMR, 13C NMR, Heteronuclear Multiple Bond Correlation (HMBC), Heteronuclear Single Quantum Coherence (HSQC), and Nuclear Overhauser Enhancement Spectroscopy (NOESY). © 2008 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.
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作者单位: Chinese Acad Sci, Xinjiang Tech Inst Phys & Chem, Xinjiang Key Lab Plant Resources & Nat Prod Chem, Urumqi 830011, Peoples R China;Chinese Acad Sci, Grad Univ, Beijing, Peoples R China;Acad Sci Uzbek, Yunusov Inst Chem Plang Substances, Tashkent, Uzbekistan
Su Zhen,Wu Hankui,Aisa Haji Akber,et al. Preparative isolation of guaipyridine sesquiterpene alkaloid from Artemisia rupestris L. flowers using high-speed counter-current chromatography[J]. Journal of Separation Science,2008,31(12):2161-2166.