黑曲霉脂肪酶的耦合固定化及特性
Alternative Titleco-immobilization of lipase from aspergillus niger and its characters
刘勇; 吾满江·艾力; 夏木西卡玛尔; 张永学; 甘争艳; 孙燕
2006
Source Publication分子催化
ISSN1001-3555
Volume20Issue:3Pages:260-266
Abstract

研究了吸附-絮凝耦合的方法固定脂肪酶的工艺条件.结果表明:在33℃下,用0.03mol/L的磷酸二氢钾?氢氧化钠缓冲液控制体系pH为7.0,酶与树脂(质量比1∶8)作用吸附1h后,用0.2mL絮凝剂聚丙烯酰胺(w(PAM)=0.5%)处理,得到活力较高的固定化脂肪酶.固定化酶最适pH9.0,最适温度为45℃,活力为405U/g,酶活回收率可以达到40%.固定化脂肪酶制备简便,可重复使用,稳定性较高.

Keyword脂肪酶 耦合固定化 吸附-絮凝 树脂 聚丙烯酰胺
Indexed ByCSCD
CSCD IDCSCD:2410910
Citation statistics
Cited Times:7[CSCD]   [CSCD Record]
Document Type期刊论文
Identifierhttp://ir.xjipc.cas.cn/handle/365002/1925
Collection新疆维吾尔自治区精细化工工程技术研究中心
Affiliation中国科学院新疆理化技术研究所;新疆大学化学化工学院;中国科学院研究生院
Recommended Citation
GB/T 7714
刘勇,吾满江·艾力,夏木西卡玛尔,等. 黑曲霉脂肪酶的耦合固定化及特性[J]. 分子催化,2006,20(3):260-266.
APA 刘勇,吾满江·艾力,夏木西卡玛尔,张永学,甘争艳,&孙燕.(2006).黑曲霉脂肪酶的耦合固定化及特性.分子催化,20(3),260-266.
MLA 刘勇,et al."黑曲霉脂肪酶的耦合固定化及特性".分子催化 20.3(2006):260-266.
Files in This Item:
File Name/Size DocType Version Access License
黑曲霉脂肪酶的耦合固定化及特性.pdf(324KB)期刊论文作者接受稿开放获取CC BY-NC-SAView Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[刘勇]'s Articles
[吾满江·艾力]'s Articles
[夏木西卡玛尔]'s Articles
Baidu academic
Similar articles in Baidu academic
[刘勇]'s Articles
[吾满江·艾力]'s Articles
[夏木西卡玛尔]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[刘勇]'s Articles
[吾满江·艾力]'s Articles
[夏木西卡玛尔]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: 黑曲霉脂肪酶的耦合固定化及特性.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.