Flat-twisted tubing: Novel column design for spiral high-speed counter-current chromatography
Yang Yi; Aisa Haji Akber; Ito Yoichiro
2009
发表期刊JOURNAL OF CHROMATOGRAPHY A
ISSN0021-9673
卷号1216期号:27页码:5265-5271
摘要The original spiral tube assembly for high-speed counter-current chromatography (HSCCC) is further improved by a new tube configuration called "flat-twisted tubing" which was made by extruding the tube (1.6 mm 1.D.) through a narrow slot followed by twisting along its axis forming about I cm twisted screw pitch. This modification interrupts the laminar How of the mobile phase through the tube and continuously mixes the two phases through the column. The performance of this spiral tube assembly was tested by three types of two-phase solvent systems with different polarities each with a set of suitable test samples such as DNP-amino acids, dipeptides and proteins at the optimal elution modes. In general all these test samples yielded higher resolution with the lower mobile phase than the upper mobile phase. In the most hydrophobic two-phase solvent system composed of hexane-ethyl acetate-methanol-0.1 M hydrochloric acid (1:1:1:1, v/v/v/v), DNP-amino acids were separated with Rs-a (peak resolution based on the same Column capacity adjusted for comparison) at 4.40 and 73% of stationary phase retention at a flow rate of 0.5 ml/min with the lower mobile phase. In the polar solvent system composed of 1 butanol-acetic acid-water (4:1:5, v/v/v), dipeptide samples were resolved with Rs-a at 4.06, compared to 2.79 with the cross-pressed tube assembly at 45% stationary phase retention, each at a flow rate of 1 ml/min. Finally in the aqueous-aqueous polymer phase systems composed of polyethylene glycol 1000 - dibasic potassium phosphate each 12.5% (w/w) in water, protein samples were resolved with Rs-a at 2.53 compared to 1.10 with the cross-pressed tube assembly at 52% of stationary phase retention,each at a now rate of 1 ml/min. These results indicate that the present system substantially improves the partition efficiency with a satisfactory level of stationary phase retention by the lower mobile phase.
关键词Spiral Tube Assembly Flat-twisted Tubing High-speed Counter-current Chromatography Retention Of The Stationary Phase Resolution Separation Of Dnp-amino Acid Dipeptide And Protein
学科领域Biochemistry & Molecular Biology ; Chemistry
DOI10.1016/j.chroma.2009.05.024
WOS记录号WOS:000267559800014
引用统计
被引频次:25[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.xjipc.cas.cn/handle/365002/1884
专题省部共建新疆特有药用资源利用重点实验室
作者单位NHLBI, Bioseparat Technol Lab, Biochem & Biophys Ctr, NIH, Bethesda, MD 20892 USA;Chinese Acad Sci, Xinjiang Tech Inst Phys & Chem, Xinjiang Key Lab Plant Resources & Nat Prod Chem, Urumqi 830011, Peoples R China;Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
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Yang Yi,Aisa Haji Akber,Ito Yoichiro. Flat-twisted tubing: Novel column design for spiral high-speed counter-current chromatography[J]. JOURNAL OF CHROMATOGRAPHY A,2009,1216(27):5265-5271.
APA Yang Yi,Aisa Haji Akber,&Ito Yoichiro.(2009).Flat-twisted tubing: Novel column design for spiral high-speed counter-current chromatography.JOURNAL OF CHROMATOGRAPHY A,1216(27),5265-5271.
MLA Yang Yi,et al."Flat-twisted tubing: Novel column design for spiral high-speed counter-current chromatography".JOURNAL OF CHROMATOGRAPHY A 1216.27(2009):5265-5271.
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