中国科学院新疆理化技术研究所机构知识库
Advanced  
XJIPC OpenIR  > 省部共建新疆特有药用资源利用重点实验室  > 期刊论文
学科主题: Biochemistry & Molecular Biology ; Chemistry
题名: Flat-twisted tubing: Novel column design for spiral high-speed counter-current chromatography
作者: Yang Yi; Aisa Haji Akber; Ito Yoichiro
关键词: Spiral tube assembly ; Flat-twisted tubing ; High-speed counter-current chromatography ; Retention of the stationary phase ; Resolution ; Separation of DNP-amino acid, dipeptide and protein
刊名: JOURNAL OF CHROMATOGRAPHY A
发表日期: 2009
DOI: 10.1016/j.chroma.2009.05.024
卷: 1216, 期:27, 页:5265-5271
摘要: The original spiral tube assembly for high-speed counter-current chromatography (HSCCC) is further improved by a new tube configuration called "flat-twisted tubing" which was made by extruding the tube (1.6 mm 1.D.) through a narrow slot followed by twisting along its axis forming about I cm twisted screw pitch. This modification interrupts the laminar How of the mobile phase through the tube and continuously mixes the two phases through the column. The performance of this spiral tube assembly was tested by three types of two-phase solvent systems with different polarities each with a set of suitable test samples such as DNP-amino acids, dipeptides and proteins at the optimal elution modes. In general all these test samples yielded higher resolution with the lower mobile phase than the upper mobile phase. In the most hydrophobic two-phase solvent system composed of hexane-ethyl acetate-methanol-0.1 M hydrochloric acid (1:1:1:1, v/v/v/v), DNP-amino acids were separated with Rs-a (peak resolution based on the same Column capacity adjusted for comparison) at 4.40 and 73% of stationary phase retention at a flow rate of 0.5 ml/min with the lower mobile phase. In the polar solvent system composed of 1 butanol-acetic acid-water (4:1:5, v/v/v), dipeptide samples were resolved with Rs-a at 4.06, compared to 2.79 with the cross-pressed tube assembly at 45% stationary phase retention, each at a flow rate of 1 ml/min. Finally in the aqueous-aqueous polymer phase systems composed of polyethylene glycol 1000 - dibasic potassium phosphate each 12.5% (w/w) in water, protein samples were resolved with Rs-a at 2.53 compared to 1.10 with the cross-pressed tube assembly at 52% of stationary phase retention,each at a now rate of 1 ml/min. These results indicate that the present system substantially improves the partition efficiency with a satisfactory level of stationary phase retention by the lower mobile phase. Published by Elsevier B.V.
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.xjipc.cas.cn/handle/365002/1884
Appears in Collections:新疆维吾尔自治区植物资源化学重点实验室 _期刊论文

Files in This Item:
File Name/ File Size Content Type Version Access License
flat twisted tubing novel column design for spiral high speed counter current chromatography.pdf(648KB)期刊论文作者接受稿开放获取View 联系获取全文

作者单位: NHLBI, Bioseparat Technol Lab, Biochem & Biophys Ctr, NIH, Bethesda, MD 20892 USA;Chinese Acad Sci, Xinjiang Tech Inst Phys & Chem, Xinjiang Key Lab Plant Resources & Nat Prod Chem, Urumqi 830011, Peoples R China;Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China

Recommended Citation:
Yang Yi,Aisa Haji Akber,Ito Yoichiro. Flat-twisted tubing: Novel column design for spiral high-speed counter-current chromatography[J]. JOURNAL OF CHROMATOGRAPHY A,2009,1216(27):5265-5271.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Yang Yi]'s Articles
[Aisa Haji Akber]'s Articles
[Ito Yoichiro]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Yang Yi]‘s Articles
[Aisa Haji Akber]‘s Articles
[Ito Yoichiro]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
文件名: flat twisted tubing novel column design for spiral high speed counter current chromatography.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Powered by CSpace