XJIPC OpenIR  > 省部共建新疆特有药用资源利用重点实验室
One-step Separation and Purification of Rupestonic Acid and Chrysosptertin B from Artemisia rupestris L. by High-speed Counter-current Chromatography
Yang Yi; Gu Dongyu; Yili Abulimiti; Zhao Yongxin; He Dajun; Aisa Haji Akber; Aisa, HA
2010
Source PublicationPHYTOCHEMICAL ANALYSIS
ISSN0958-0344
Volume21Issue:2Pages:205-209
AbstractIntroduction - Artemisia rupestris L. is a well-known traditional Chinese medicinal plant in Xinjiang. Rupestonic acid is the main active ingredient of A. rupestris L., and has been chosen as a 'marker compound' for the chemical evaluation or quality control of A. rupestris L. and its products. Although HSCCC separation method was developed before, the separation was performed with two steps using the same solvent system, which were time-consuming and waste of the solvents. Objective - To develop a simple HSCCC method for the separation and purification of rupestonic acid in a single run. Methodology - The measurement of partition coefficient (K) was introduced to select the two-phase solvent system. The simple HSCCC method was established according to the selected solvent system for separation and purification of rupestonic acid. The purity of target compound was test by HPLC and the structure was identified by MS,(1)H NMR and (13)C NMR. Results - A total of 72.3 mg of rupestonic acid and 53.5 mg of chrysosptertin B with over 95% purity were yielded from 500 mg extracts of Artemisia rupestris L. in one-step separation. Conclusion - The rupestonic acid was separated in a single run by HSCCC. Copyright (C) 2009 John Wiley & Sons, Ltd.
KeywordHigh-speed Counter-current Chromatography Artemisia Rupestris L. Rupestonic Acid Chrysosptertin b One-step Separation
Subject AreaBiochemistry & Molecular Biology ; Plant Sciences ; Chemistry
DOI10.1002/pca.1169
Indexed BySCI
WOS IDWOS:000275007400011
Citation statistics
Cited Times:12[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.xjipc.cas.cn/handle/365002/1869
Collection省部共建新疆特有药用资源利用重点实验室
Corresponding AuthorAisa, HA
AffiliationChinese Acad Sci, Xinjiang Tech Inst Phys & Chem, Xinjiang Key Lab Plant Resources & Nat Prod Chem, Urumqi 830011, Peoples R China;Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Yang Yi,Gu Dongyu,Yili Abulimiti,et al. One-step Separation and Purification of Rupestonic Acid and Chrysosptertin B from Artemisia rupestris L. by High-speed Counter-current Chromatography[J]. PHYTOCHEMICAL ANALYSIS,2010,21(2):205-209.
APA Yang Yi.,Gu Dongyu.,Yili Abulimiti.,Zhao Yongxin.,He Dajun.,...&Aisa, HA.(2010).One-step Separation and Purification of Rupestonic Acid and Chrysosptertin B from Artemisia rupestris L. by High-speed Counter-current Chromatography.PHYTOCHEMICAL ANALYSIS,21(2),205-209.
MLA Yang Yi,et al."One-step Separation and Purification of Rupestonic Acid and Chrysosptertin B from Artemisia rupestris L. by High-speed Counter-current Chromatography".PHYTOCHEMICAL ANALYSIS 21.2(2010):205-209.
Files in This Item:
File Name/Size DocType Version Access License
one-step separation (239KB)期刊论文作者接受稿开放获取CC BY-NC-SAView Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Yang Yi]'s Articles
[Gu Dongyu]'s Articles
[Yili Abulimiti]'s Articles
Baidu academic
Similar articles in Baidu academic
[Yang Yi]'s Articles
[Gu Dongyu]'s Articles
[Yili Abulimiti]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Yang Yi]'s Articles
[Gu Dongyu]'s Articles
[Yili Abulimiti]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: one-step separation and purification of rupestonic acid and chrysosptertin b from artemisia rupestris l. by high-speed countercurrent chromatography.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.