XJIPC OpenIR  > 省部共建新疆特有药用资源利用重点实验室
Studies on the effect of column angle in centrifugal helix counter-current chromatography
Yang Yi; Gu Dongyu; Aisa Haji Akber; Ito Yoichiro
2010
Source PublicationJournal of Chromatography A
ISSN219673
Volume1217Issue:14Pages:2117-2122
AbstractThe performance of the coiled column of centrifugal counter-current chromatography was investigated by changing the angle between column axis and centrifugal force in the separation of dipeptides or DNP-amino acids each with suitable two-phase solvent systems. In general, retention of the stationary phase (Sf) decreased, and peak resolution (Rs) increased as the column angle was increased. The first series of experiments was performed using a polar two-phase solvent system composed of 1-butanol-acetic acid-water (4:1:5, v/v/v) to separate two dipeptide samples, Trp-Tyr and Val-Tyr, at a flow rate of 1 ml/min at 1000 rpm. When the column angle was changed from 0° to 90°, Rs increased from 1.05 (Sf = 60.1%) to 1.17 (Sf = 38.7%) with the lower phase mobile and from 1.02 (Sf = 67.8%) to 1.14 (Sf = 47.4%) with the upper phase mobile, respectively. The second series of experiments was similarly performed with a more hydrophobic two-phase solvent system composed of hexane-ethyl acetate-methanol-0.1 M hydrochloric acid (1:1:1:1, v/v/v/v) to separate three DNP-amino acids, DNP-glu, DNP-β-ala and DNP-ala, at a flow rate of 1 ml/min at 1000 rpm. When the column angle was changed from 0° to 90°, Rs increased from 1.38 (1st peak/2nd peak) and 1.20 (2nd peak/3rd peak) (Sf = 61.1%) to 1.66 and 1.45 (Sf = 34.4%) with the lower phase mobile and from 1.14 and 0.63 (Sf = 72.2%) to 1.53 and 0.87 (Sf = 51.1%) with the upper phase mobile, respectively. The overall results of our studies indicate that increasing the column angle against the radially acting centrifugal force enhances the mixing of two phases in the column to improve the peak while decreasing the stationary phase retention by interrupting the laminar flow of the mobile phase.
KeywordCentrifugal Counter-current Chromatography Angle Between Column Axis And Centrifugal Force Retention Of The Stationary Phase Resolution Dipeptide Dnp-amino Acid
Subject AreaBiochemistry & Molecular Biology ; Chemistry
DOI10.1016/j.chroma.2010.02.003
WOS IDWOS:000276285600006
Citation statistics
Cited Times:4[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.xjipc.cas.cn/handle/365002/1854
Collection省部共建新疆特有药用资源利用重点实验室
AffiliationNHLBI, NIH, Bioseparat Technol Lab, Biochem & Biophys Ctr, Bethesda, MD 20892 USA;Chinese Acad Sci, Xinjiang Key Lab Plant Resources & Nat Prod Chem, Xinjiang Tech Inst Phys & Chem, Urumqi 830011, Peoples R China; Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Yang Yi,Gu Dongyu,Aisa Haji Akber,et al. Studies on the effect of column angle in centrifugal helix counter-current chromatography[J]. Journal of Chromatography A,2010,1217(14):2117-2122.
APA Yang Yi,Gu Dongyu,Aisa Haji Akber,&Ito Yoichiro.(2010).Studies on the effect of column angle in centrifugal helix counter-current chromatography.Journal of Chromatography A,1217(14),2117-2122.
MLA Yang Yi,et al."Studies on the effect of column angle in centrifugal helix counter-current chromatography".Journal of Chromatography A 1217.14(2010):2117-2122.
Files in This Item:
File Name/Size DocType Version Access License
studies on the effec(1060KB)期刊论文作者接受稿开放获取CC BY-NC-SAView Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Yang Yi]'s Articles
[Gu Dongyu]'s Articles
[Aisa Haji Akber]'s Articles
Baidu academic
Similar articles in Baidu academic
[Yang Yi]'s Articles
[Gu Dongyu]'s Articles
[Aisa Haji Akber]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Yang Yi]'s Articles
[Gu Dongyu]'s Articles
[Aisa Haji Akber]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: studies on the effect of column angle in centrifugal helix countercurrent chromatography.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.