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学科主题: Biochemistry & Molecular Biology ; Chemistry
题名: Studies on the effect of column angle in centrifugal helix counter-current chromatography
作者: Yang Yi; Gu Dongyu; Aisa Haji Akber; Ito Yoichiro
关键词: Centrifugal counter-current chromatography ; Angle between column axis and centrifugal force ; Retention of the stationary phase ; Resolution ; Dipeptide ; DNP-amino acid
刊名: Journal of Chromatography A
发表日期: 2010
DOI: 10.1016/j.chroma.2010.02.003
卷: 1217, 期:14, 页:2117-2122
摘要: The performance of the coiled column of centrifugal counter-current chromatography was investigated by changing the angle between column axis and centrifugal force in the separation of dipeptides or DNP-amino acids each with suitable two-phase solvent systems. In general, retention of the stationary phase (Sf) decreased, and peak resolution (Rs) increased as the column angle was increased. The first series of experiments was performed using a polar two-phase solvent system composed of 1-butanol-acetic acid-water (4:1:5, v/v/v) to separate two dipeptide samples, Trp-Tyr and Val-Tyr, at a flow rate of 1 ml/min at 1000 rpm. When the column angle was changed from 0° to 90°, Rs increased from 1.05 (Sf = 60.1%) to 1.17 (Sf = 38.7%) with the lower phase mobile and from 1.02 (Sf = 67.8%) to 1.14 (Sf = 47.4%) with the upper phase mobile, respectively. The second series of experiments was similarly performed with a more hydrophobic two-phase solvent system composed of hexane-ethyl acetate-methanol-0.1 M hydrochloric acid (1:1:1:1, v/v/v/v) to separate three DNP-amino acids, DNP-glu, DNP-β-ala and DNP-ala, at a flow rate of 1 ml/min at 1000 rpm. When the column angle was changed from 0° to 90°, Rs increased from 1.38 (1st peak/2nd peak) and 1.20 (2nd peak/3rd peak) (Sf = 61.1%) to 1.66 and 1.45 (Sf = 34.4%) with the lower phase mobile and from 1.14 and 0.63 (Sf = 72.2%) to 1.53 and 0.87 (Sf = 51.1%) with the upper phase mobile, respectively. The overall results of our studies indicate that increasing the column angle against the radially acting centrifugal force enhances the mixing of two phases in the column to improve the peak while decreasing the stationary phase retention by interrupting the laminar flow of the mobile phase.
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内容类型: 期刊论文
URI标识: http://ir.xjipc.cas.cn/handle/365002/1854
Appears in Collections:新疆维吾尔自治区植物资源化学重点实验室 _期刊论文

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作者单位: NHLBI, NIH, Bioseparat Technol Lab, Biochem & Biophys Ctr, Bethesda, MD 20892 USA;Chinese Acad Sci, Xinjiang Key Lab Plant Resources & Nat Prod Chem, Xinjiang Tech Inst Phys & Chem, Urumqi 830011, Peoples R China; Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China

Recommended Citation:
Yang Yi,Gu Dongyu,Aisa Haji Akber,et al. Studies on the effect of column angle in centrifugal helix counter-current chromatography[J]. Journal of Chromatography A,2010,1217(14):2117-2122.
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