Studies on the effect of column angle in centrifugal helix counter-current chromatography
Yang Yi; Gu Dongyu; Aisa Haji Akber; Ito Yoichiro
2010
发表期刊Journal of Chromatography A
ISSN219673
卷号1217期号:14页码:2117-2122
摘要The performance of the coiled column of centrifugal counter-current chromatography was investigated by changing the angle between column axis and centrifugal force in the separation of dipeptides or DNP-amino acids each with suitable two-phase solvent systems. In general, retention of the stationary phase (Sf) decreased, and peak resolution (Rs) increased as the column angle was increased. The first series of experiments was performed using a polar two-phase solvent system composed of 1-butanol-acetic acid-water (4:1:5, v/v/v) to separate two dipeptide samples, Trp-Tyr and Val-Tyr, at a flow rate of 1 ml/min at 1000 rpm. When the column angle was changed from 0° to 90°, Rs increased from 1.05 (Sf = 60.1%) to 1.17 (Sf = 38.7%) with the lower phase mobile and from 1.02 (Sf = 67.8%) to 1.14 (Sf = 47.4%) with the upper phase mobile, respectively. The second series of experiments was similarly performed with a more hydrophobic two-phase solvent system composed of hexane-ethyl acetate-methanol-0.1 M hydrochloric acid (1:1:1:1, v/v/v/v) to separate three DNP-amino acids, DNP-glu, DNP-β-ala and DNP-ala, at a flow rate of 1 ml/min at 1000 rpm. When the column angle was changed from 0° to 90°, Rs increased from 1.38 (1st peak/2nd peak) and 1.20 (2nd peak/3rd peak) (Sf = 61.1%) to 1.66 and 1.45 (Sf = 34.4%) with the lower phase mobile and from 1.14 and 0.63 (Sf = 72.2%) to 1.53 and 0.87 (Sf = 51.1%) with the upper phase mobile, respectively. The overall results of our studies indicate that increasing the column angle against the radially acting centrifugal force enhances the mixing of two phases in the column to improve the peak while decreasing the stationary phase retention by interrupting the laminar flow of the mobile phase.
关键词Centrifugal Counter-current Chromatography Angle Between Column Axis And Centrifugal Force Retention Of The Stationary Phase Resolution Dipeptide Dnp-amino Acid
学科领域Biochemistry & Molecular Biology ; Chemistry
DOI10.1016/j.chroma.2010.02.003
WOS记录号WOS:000276285600006
引用统计
被引频次:4[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.xjipc.cas.cn/handle/365002/1854
专题省部共建新疆特有药用资源利用重点实验室
作者单位NHLBI, NIH, Bioseparat Technol Lab, Biochem & Biophys Ctr, Bethesda, MD 20892 USA;Chinese Acad Sci, Xinjiang Key Lab Plant Resources & Nat Prod Chem, Xinjiang Tech Inst Phys & Chem, Urumqi 830011, Peoples R China; Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
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Yang Yi,Gu Dongyu,Aisa Haji Akber,et al. Studies on the effect of column angle in centrifugal helix counter-current chromatography[J]. Journal of Chromatography A,2010,1217(14):2117-2122.
APA Yang Yi,Gu Dongyu,Aisa Haji Akber,&Ito Yoichiro.(2010).Studies on the effect of column angle in centrifugal helix counter-current chromatography.Journal of Chromatography A,1217(14),2117-2122.
MLA Yang Yi,et al."Studies on the effect of column angle in centrifugal helix counter-current chromatography".Journal of Chromatography A 1217.14(2010):2117-2122.
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