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学科主题: Biochemistry & Molecular Biology ; Chemistry
题名: Compact type-I coil planet centrifuge for counter-current chromatography
作者: Yang Yi; Gu Dongyu; Liu Yongqiang; Aisa Haji Akber; Ito Yoichiro
关键词: Compact type-I coil planet centrifuge ; Counter-current chromatography ; DNP-amino acids ; Revolution radius ; Retention of the stationary phase ; Resolution ; Retention time
刊名: Journal of Chromatography A
发表日期: 2010
DOI: 10.1016/j.chroma.2009.12.055
卷: 1217, 期:8, 页:1313-1319
英文摘要: A compact type-I coil planet centrifuge has been developed for performing counter-current chromatography. It has a revolution radius of 10 cm and a column holder height of 5 cm compared with 37 and 50 cm in the original prototype, respectively. The reduction in the revolution radius and column length permits application of higher revolution speed and more stable balancing of the rotor which leads us to learn more about its performance and the future potential of type-I coil planet centrifuge. The chromatographic performance of this apparatus was evaluated in terms of retention of the stationary phase (Sf), peak resolution (Rs), theoretical plate (N) and peak retention time (tR). The results of the experiment indicated that increasing the revolution speed slightly improved both the retention of the stationary phase and the peak resolution while the separation time is remarkably shortened to yield an excellent peak resolution at a revolution speed of 800 rpm. With a 12 ml capacity coiled column, DNP-DL-glu, DNP-β-ala and DNP-l-ala were resolved at Rs of 2.75 and 2.16 within 90 min at a flow rate of 0.4 ml/min. We believe that the compact type-I coil planet centrifuge has a high analytical potential.
WOS记录号: WOS:000274953400017
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内容类型: 期刊论文
URI标识: http://ir.xjipc.cas.cn/handle/365002/1849
Appears in Collections:新疆维吾尔自治区植物资源化学重点实验室 _期刊论文

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作者单位: NHLBI, Bioseparat Technol Lab, Biochem & Biophys Ctr, NIH, Bethesda, MD 20892 USA;Chinese Acad Sci, Xinjiang Tech Inst Phys & Chem, Xinjiang Key Lab Plant Resources & Nat Prod Chem, Urumqi 830011, Peoples R China;Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China

Recommended Citation:
Yang Yi,Gu Dongyu,Liu Yongqiang,et al. Compact type-I coil planet centrifuge for counter-current chromatography[J]. Journal of Chromatography A,2010,1217(8):1313-1319.
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