Compact type-I coil planet centrifuge for counter-current chromatography
Yang Yi; Gu Dongyu; Liu Yongqiang; Aisa Haji Akber; Ito Yoichiro
2010
发表期刊Journal of Chromatography A
ISSN219673
卷号1217期号:8页码:1313-1319
摘要A compact type-I coil planet centrifuge has been developed for performing counter-current chromatography. It has a revolution radius of 10 cm and a column holder height of 5 cm compared with 37 and 50 cm in the original prototype, respectively. The reduction in the revolution radius and column length permits application of higher revolution speed and more stable balancing of the rotor which leads us to learn more about its performance and the future potential of type-I coil planet centrifuge. The chromatographic performance of this apparatus was evaluated in terms of retention of the stationary phase (Sf), peak resolution (Rs), theoretical plate (N) and peak retention time (tR). The results of the experiment indicated that increasing the revolution speed slightly improved both the retention of the stationary phase and the peak resolution while the separation time is remarkably shortened to yield an excellent peak resolution at a revolution speed of 800 rpm. With a 12 ml capacity coiled column, DNP-DL-glu, DNP-β-ala and DNP-l-ala were resolved at Rs of 2.75 and 2.16 within 90 min at a flow rate of 0.4 ml/min. We believe that the compact type-I coil planet centrifuge has a high analytical potential.
关键词Compact Type-i Coil Planet Centrifuge Counter-current Chromatography Dnp-amino Acids Revolution Radius Retention Of The Stationary Phase Resolution Retention Time
学科领域Biochemistry & Molecular Biology ; Chemistry
DOI10.1016/j.chroma.2009.12.055
WOS记录号WOS:000274953400017
引用统计
被引频次:9[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.xjipc.cas.cn/handle/365002/1849
专题省部共建新疆特有药用资源利用重点实验室
作者单位NHLBI, Bioseparat Technol Lab, Biochem & Biophys Ctr, NIH, Bethesda, MD 20892 USA;Chinese Acad Sci, Xinjiang Tech Inst Phys & Chem, Xinjiang Key Lab Plant Resources & Nat Prod Chem, Urumqi 830011, Peoples R China;Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
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GB/T 7714
Yang Yi,Gu Dongyu,Liu Yongqiang,et al. Compact type-I coil planet centrifuge for counter-current chromatography[J]. Journal of Chromatography A,2010,1217(8):1313-1319.
APA Yang Yi,Gu Dongyu,Liu Yongqiang,Aisa Haji Akber,&Ito Yoichiro.(2010).Compact type-I coil planet centrifuge for counter-current chromatography.Journal of Chromatography A,1217(8),1313-1319.
MLA Yang Yi,et al."Compact type-I coil planet centrifuge for counter-current chromatography".Journal of Chromatography A 1217.8(2010):1313-1319.
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