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Separation and Purification of Phenolic Acids and Myricetin from Black Currant by High-Speed Countercurrent Chromatography
He Dajun; Gu Dongyu; Huang Yun; Ayupbek Amatjan; Yang Yi; Aisa Haji Akber; Ito Yoichiro
2009
Source PublicationJOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
ISSN1082-6076
Volume32Issue:20Pages:3077-3088
Abstract

Black currant is an important material in the food industry, but little research has been reported on the isolation of phenolic acids because of their low content. In the present study, high-speed countercurrent chromatography (HSCCC) has been successfully used for the preparative isolation of the minor phenolic compounds from the ethyl acetate extracts of black currant fruit. The HSCCC separation was performed with a two phase solvent system composed of n-hexane/EtOAc/MeOH/H2O (5:15:4:7v/v) at a flow rate of 1.5mL/min. From a 500mg crude sample 0.8mg of protocatechuic acid, 1.0mg of caffeic acid, 0.5mg of 4-hydroxybenzoic acid, and 2.5mg of myricetin were purified by a one step HSCCC operation,. Their chemical structures were confirmed by MS and NMR.

KeywordBlack Currant Caffeic Acid High-speed Countercurrent Chromatography (Hsccc) 4-hydroxybenzoic Acid Myricetin Protocatechuic Acid
Subject AreaBiochemistry & Molecular Biology ; Chemistry
DOI10.1080/10826070903320756
Indexed BySCI
WOS IDWOS:000271822900013
Citation statistics
Document Type期刊论文
Identifierhttp://ir.xjipc.cas.cn/handle/365002/1848
Collection省部共建新疆特有药用资源利用重点实验室
资源化学研究室
Affiliation1.NHLBI, Bioseparat Technol Lab, Biochem & Biophys Ctr, NIH, Bethesda, MD 20892 USA
2.Chinese Acad Sci, Xinjiang Tech Inst Phys & Chem, Xinjiang Key Lab Plant Resources & Nat Prod Chem, Urumqi 830011, Peoples R China
3.Union Labs Bioact Ingredients Edible Plants Cent, Urumqi, Peoples R China
4.Shihezi Univ, Sch Life Sci, Shihezi, Peoples R China
5.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China
Recommended Citation
GB/T 7714
He Dajun,Gu Dongyu,Huang Yun,et al. Separation and Purification of Phenolic Acids and Myricetin from Black Currant by High-Speed Countercurrent Chromatography[J]. JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,2009,32(20):3077-3088.
APA He Dajun.,Gu Dongyu.,Huang Yun.,Ayupbek Amatjan.,Yang Yi.,...&Ito Yoichiro.(2009).Separation and Purification of Phenolic Acids and Myricetin from Black Currant by High-Speed Countercurrent Chromatography.JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,32(20),3077-3088.
MLA He Dajun,et al."Separation and Purification of Phenolic Acids and Myricetin from Black Currant by High-Speed Countercurrent Chromatography".JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES 32.20(2009):3077-3088.
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