Separation and Purification of Phenolic Acids and Myricetin from Black Currant by High-Speed Countercurrent Chromatography
He Dajun; Gu Dongyu; Huang Yun; Ayupbek Amatjan; Yang Yi; Aisa Haji Akber; Ito Yoichiro
2009
发表期刊JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
ISSN10826076
卷号32期号:20页码:3077-3088
摘要Black currant is an important material in the food industry, but little research has been reported on the isolation of phenolic acids because of their low content. In the present study, high-speed countercurrent chromatography (HSCCC) has been successfully used for the preparative isolation of the minor phenolic compounds from the ethyl acetate extracts of black currant fruit. The HSCCC separation was performed with a two phase solvent system composed of n-hexane/EtOAc/MeOH/H2O (5:15:4:7v/v) at a flow rate of 1.5mL/min. From a 500mg crude sample 0.8mg of protocatechuic acid, 1.0mg of caffeic acid, 0.5mg of 4-hydroxybenzoic acid, and 2.5mg of myricetin were purified by a one step HSCCC operation,. Their chemical structures were confirmed by MS and NMR.
关键词Black Currant Caffeic Acid High-speed Countercurrent Chromatography (Hsccc) 4-hydroxybenzoic Acid Myricetin Protocatechuic Acid
学科领域Biochemistry & Molecular Biology ; Chemistry
DOI10.1080/10826070903320756
WOS记录号WOS:000271822900013
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文献类型期刊论文
条目标识符http://ir.xjipc.cas.cn/handle/365002/1848
专题省部共建新疆特有药用资源利用重点实验室
作者单位NHLBI, Bioseparat Technol Lab, Biochem & Biophys Ctr, NIH, Bethesda, MD 20892 USA;Chinese Acad Sci, Xinjiang Tech Inst Phys & Chem, Xinjiang Key Lab Plant Resources & Nat Prod Chem, Urumqi 830011, Peoples R China;Union Labs Bioact Ingredients Edible Plants Cent, Urumqi, Peoples R China;Shihezi Univ, Sch Life Sci, Shihezi, Peoples R China;Chinese Acad Sci, Grad Univ, Beijing, Peoples R China
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He Dajun,Gu Dongyu,Huang Yun,et al. Separation and Purification of Phenolic Acids and Myricetin from Black Currant by High-Speed Countercurrent Chromatography[J]. JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,2009,32(20):3077-3088.
APA He Dajun.,Gu Dongyu.,Huang Yun.,Ayupbek Amatjan.,Yang Yi.,...&Ito Yoichiro.(2009).Separation and Purification of Phenolic Acids and Myricetin from Black Currant by High-Speed Countercurrent Chromatography.JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,32(20),3077-3088.
MLA He Dajun,et al."Separation and Purification of Phenolic Acids and Myricetin from Black Currant by High-Speed Countercurrent Chromatography".JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES 32.20(2009):3077-3088.
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