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学科主题: Biochemistry & Molecular Biology ; Chemistry
题名: Separation and Purification of Phenolic Acids and Myricetin from Black Currant by High-Speed Countercurrent Chromatography
作者: He Dajun; Gu Dongyu; Huang Yun; Ayupbek Amatjan; Yang Yi; Aisa Haji Akber; Ito Yoichiro
关键词: Black currant ; Caffeic acid ; High-speed countercurrent chromatography (HSCCC) ; 4-Hydroxybenzoic acid ; Myricetin ; Protocatechuic acid
刊名: Journal of Liquid Chromatography and Related Technologies
发表日期: 2009
DOI: 10.1080/10826070903320756
卷: 32, 期:20, 页:3077-3088
资助者: Chinese Academy of Sciences State Administration of Foreign Experts Affairs
摘要: Black currant is an important material in the food industry, but little research has been reported on the isolation of phenolic acids because of their low content. In the present study, high-speed countercurrent chromatography (HSCCC) has been successfully used for the preparative isolation of the minor phenolic compounds from the ethyl acetate extracts of black currant fruit. The HSCCC separation was performed with a two phase solvent system composed of n-hexane/EtOAc/MeOH/H2O (5:15:4:7v/v) at a flow rate of 1.5mL/min. From a 500mg crude sample 0.8mg of protocatechuic acid, 1.0mg of caffeic acid, 0.5mg of 4-hydroxybenzoic acid, and 2.5mg of myricetin were purified by a one step HSCCC operation,. Their chemical structures were confirmed by MS and NMR.
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内容类型: 期刊论文
URI标识: http://ir.xjipc.cas.cn/handle/365002/1848
Appears in Collections:新疆维吾尔自治区植物资源化学重点实验室 _期刊论文

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作者单位: NHLBI, Bioseparat Technol Lab, Biochem & Biophys Ctr, NIH, Bethesda, MD 20892 USA;Chinese Acad Sci, Xinjiang Tech Inst Phys & Chem, Xinjiang Key Lab Plant Resources & Nat Prod Chem, Urumqi 830011, Peoples R China;Union Labs Bioact Ingredients Edible Plants Cent, Urumqi, Peoples R China;Shihezi Univ, Sch Life Sci, Shihezi, Peoples R China;Chinese Acad Sci, Grad Univ, Beijing, Peoples R China

Recommended Citation:
He Dajun,Gu Dongyu,Huang Yun,et al. Separation and Purification of Phenolic Acids and Myricetin from Black Currant by High-Speed Countercurrent Chromatography[J]. Journal of Liquid Chromatography and Related Technologies,2009,32(20):3077-3088.
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文件名: separation and purification of phenolic acids and myricetin from black currant by high speed countercurrent chromatography.pdf
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