中国科学院新疆理化技术研究所机构知识库
Advanced  
XJIPC OpenIR  > 省部共建新疆特有药用资源利用重点实验室  > 期刊论文
学科主题: Biochemistry & Molecular Biology ; Chemistry
题名: Mathematical model of computer-programmed intermittent dual countercurrent chromatography applied to hydrostatic and hydrodynamic equilibrium systems
作者: Yang Yi; Aisa Haji Akber; Ito Yoichiro
关键词: Programmed intermittent dual countercurrent chromatography ; Mathematical model ; Spiral tube assembly ; Toroidal coil countercurrent chromatography ; Sliding valve ; Hydrostatic and hydrodynamic equilibrium systems
刊名: Journal of Chromatography A
发表日期: 2009
DOI: 10.1016/j.chroma.2009.07.015
卷: 1216, 期:35, 页:6310-6318
摘要: Dual high-speed countercurrent chromatography (dual CCC) literally permits countercurrent flow of two immiscible solvent phases continuously through the coiled column for separation of solutes according to their partition coefficients. Application of this technique has been successfully demonstrated by separation of analytes by gas-liquid and liquid-liquid two-phase systems. However, the method cannot be directly applied to the system with a set of coiled columns connected in series, since the countercurrent process is interrupted at the junction between the columns. However, this problem can be solved by intermittent dual CCC by eluting each phase alternately through the opposite ends of the separation column. This mode of application has an advantage over the conventional dual CCC in that the method can be applied to all types of CCC systems including hydrostatic equilibrium systems such as toroidal coil CCC and centrifugal partition chromatography. Recently, the application of this method to high-speed CCC (hydrodynamic system) has been demonstrated for separation of natural products by Hewitson et al. using a set of conventional multilayer coil separation columns connected in series. Here, we have developed a mathematical model for this intermittent dual CCC system to predict retention time of the analytes, and using a simplified model system the validity of the model is justified by a series of basic studies on both hydrodynamic and hydrostatic CCC systems with a computer-programmed single sliding valve. The present method has been successfully applied to spiral tube assembly high-speed CCC (hydrodynamic system) and toroidal coil CCC (hydrostatic system) for separation of DNP-amino acid samples with two biphasic solvent systems composed of hexane-ethyl acetate-methanol-0.1 M hydrochloric acid (1:1:1:1 and 4:5:4:5, v/v).
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.xjipc.cas.cn/handle/365002/1836
Appears in Collections:新疆维吾尔自治区植物资源化学重点实验室 _期刊论文

Files in This Item:
File Name/ File Size Content Type Version Access License
mathematical model of computerprogrammed intermittent dual countercurrent chromatography applied to hydrostatic and hydrodynamic equilibrium systems.pdf(488KB)期刊论文作者接受稿开放获取View 联系获取全文

作者单位: NHLBI, Bioseparat Technol Lab, Biochem & Biophys Ctr, NIH, Bethesda, MD 20892 USA;Chinese Acad Sci, Xinjiang Key Lab Plant Resources & Nat Prod Chem, Xinjiang Tech Inst Phys & Chem, Urumqi 830011, Peoples R China;Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China

Recommended Citation:
Yang Yi,Aisa Haji Akber,Ito Yoichiro. Mathematical model of computer-programmed intermittent dual countercurrent chromatography applied to hydrostatic and hydrodynamic equilibrium systems[J]. Journal of Chromatography A,2009,1216(35):6310-6318.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Yang Yi]'s Articles
[Aisa Haji Akber]'s Articles
[Ito Yoichiro]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Yang Yi]‘s Articles
[Aisa Haji Akber]‘s Articles
[Ito Yoichiro]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
文件名: mathematical model of computerprogrammed intermittent dual countercurrent chromatography applied to hydrostatic and hydrodynamic equilibrium systems.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Powered by CSpace