SEPARATION AND PURIFICATION OF FLAVONOIDS FROM BLACK CURRANT LEAVES BY HIGH-SPEED COUNTERCURRENT CHROMATOGRAPHY AND PREPARATIVE HPLC
He Dajun; Huang Yun; Ayupbek Amatjan; Gu Dongyu; Yang Yi; Aisa Haji Akber; Ito Yoichiro; Aisa, HA
2010
发表期刊JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
ISSN10826076
卷号33期号:5页码:615-628
摘要High-speed countercurrent chromatography (HSCCC) has been successfully used for the preparative isolation of flavonoids from the ethyl acetate extracts of black currant leaves. The HSCCC separation was performed with a two-phase solvent system composed of n-hexane/EtOAc/MeOH/H2O (1:10:1:10, v/v) at a flow rate of 1.5mL/min. When the flow rate was increased from 1.0 to 3.0mL/min, the retention of stationary phase decreased from 60.3% to 39.7% resulting in a loss of peak resolution, while the stationary phase retention is stable with an increase in sample size from 25 to 200mg. From 100mg of the crude sample, HSCCC separation yielded 4.0mg of kaempferol 3-O-galactoside, 6.0mg of kaempferol 3-O-glucoside, and 9.0mg of fraction I containing a mixture of hyperoside and isoquercitrin. Then, from 18mg of fraction I, 3.0mg of hyperoside and 11.0mg isoquercitrin were separated by preparative HPLC by successive sample injection at every 100min interval. Chemical structures of all these compounds were confirmed by MS and NMR.
关键词Black Currant High-speed Countercurrent Chromatography (Hsccc) Hyperoside Isoquercitrin Kaempferol 3-o-galactoside Kaempferol 3-o-glucoside
学科领域Biochemistry & Molecular Biology ; Chemistry
DOI10.1080/10826071003608447
收录类别SCI
WOS记录号WOS:000275112200003
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被引频次:27[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.xjipc.cas.cn/handle/365002/1787
专题省部共建新疆特有药用资源利用重点实验室
通讯作者Aisa, HA
作者单位Chinese Acad Sci, Xinjiang Tech Inst Phys & Chem, Xinjiang Key Lab Plant Resources & Nat Prod Chem, Urumqi 830011, Peoples R China;Shihezi Univ, Sch Life Sci, Shihezi, Peoples R China;Chinese Acad Sci, Grad Univ, Beijing, Peoples R China;NHLBI, Bioseparat Technol Lab, Biochem & Biophys Ctr, NIH, Bethesda, MD 20892 USA
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He Dajun,Huang Yun,Ayupbek Amatjan,et al. SEPARATION AND PURIFICATION OF FLAVONOIDS FROM BLACK CURRANT LEAVES BY HIGH-SPEED COUNTERCURRENT CHROMATOGRAPHY AND PREPARATIVE HPLC[J]. JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,2010,33(5):615-628.
APA He Dajun.,Huang Yun.,Ayupbek Amatjan.,Gu Dongyu.,Yang Yi.,...&Aisa, HA.(2010).SEPARATION AND PURIFICATION OF FLAVONOIDS FROM BLACK CURRANT LEAVES BY HIGH-SPEED COUNTERCURRENT CHROMATOGRAPHY AND PREPARATIVE HPLC.JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,33(5),615-628.
MLA He Dajun,et al."SEPARATION AND PURIFICATION OF FLAVONOIDS FROM BLACK CURRANT LEAVES BY HIGH-SPEED COUNTERCURRENT CHROMATOGRAPHY AND PREPARATIVE HPLC".JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES 33.5(2010):615-628.
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