SEPARATION OF THE MINOR FLAVONOLS FROM FLOS GOSSYPII BY HIGH-SPEED COUNTERCURRENT CHROMATOGRAPHY
Yang Yi; Zhao Yongxin; Gu Dongyu; Ayupbek Amatjan; Huang Yun; Dou Jun; Ito Yoichiro; Zhang Tianyou; Aisa Haji Akber
2010
发表期刊JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
ISSN10826076
卷号33期号:16页码:1502-1515
摘要An effective high-speed countercurrent chromatography (HSCCC) method was established for further separation and purification of four minor flavonols in addition to five major flavonols which were reported by our previous study from extracts of Flos gossypii. HSCCC was performed with three two-phase solvent systems composed of n-hexane-ethyl acetate-methanol-water (7.5:15:6:7, v/v), (2.5:15:2:7, v/v), and (0:1:0:1, v/v). The separation was repeated three times, and 3.8mg of 8-methoxyl-kaempferol-7-O-β-D-rhamnoside (HPLC purity 98.27%), 6.7mg of astragalin (HPLC purity 94.18%), 3.3mg of 4'-methoxyl-quercetin-7-O- β-D-glucoside (HPLC purity 94.30%), and 8.2mg of hyperoside (HPLC purity 93.48%) were separated from 150mg of the crude sample. The chemical structures of the flavonols were confirmed by MS, 1H NMR, and 13C NMR. Meanwhile, the results indicated that the target compound with smaller K value (<0.5) can be separated by increasing column length of HSCCC. And, four separation rules of flavonols were established according to the present study and references were summarized, which can be used as a useful guide for separation of flavonols by HSCCC.
关键词Flavonol Flos Gossypii Hsccc Separation Rules Of Flavonols
学科领域Biochemistry & Molecular Biology ; Chemistry
DOI10.1080/10826076.2010.489000
收录类别SCI
WOS记录号WOS:000282581800002
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被引频次:10[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.xjipc.cas.cn/handle/365002/1786
专题省部共建新疆特有药用资源利用重点实验室
作者单位Biochem & Biophys Ctr, Bioseparat Technol Lab, NHLBI, NIH, Bethesda, MD 20892 USA;Chinese Acad Sci, Xinjiang Key Lab Plant Resources & Nat Prod Chem, Xinjiang Tech Inst Phys & Chem, Urumqi, Peoples R China;Chinese Acad Sci, Key Lab Chem Plant Resources Arid Area, Xinjiang Tech Inst Phys & Chem, Urumqi, Peoples R China;Beijing Inst New Technol Applicat, Beijing, Peoples R China
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Yang Yi,Zhao Yongxin,Gu Dongyu,et al. SEPARATION OF THE MINOR FLAVONOLS FROM FLOS GOSSYPII BY HIGH-SPEED COUNTERCURRENT CHROMATOGRAPHY[J]. JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,2010,33(16):1502-1515.
APA Yang Yi.,Zhao Yongxin.,Gu Dongyu.,Ayupbek Amatjan.,Huang Yun.,...&Aisa Haji Akber.(2010).SEPARATION OF THE MINOR FLAVONOLS FROM FLOS GOSSYPII BY HIGH-SPEED COUNTERCURRENT CHROMATOGRAPHY.JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,33(16),1502-1515.
MLA Yang Yi,et al."SEPARATION OF THE MINOR FLAVONOLS FROM FLOS GOSSYPII BY HIGH-SPEED COUNTERCURRENT CHROMATOGRAPHY".JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES 33.16(2010):1502-1515.
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