XJIPC OpenIR  > 省部共建新疆特有药用资源利用重点实验室
SEPARATION OF THE MINOR FLAVONOLS FROM FLOS GOSSYPII BY HIGH-SPEED COUNTERCURRENT CHROMATOGRAPHY
Yang Yi; Zhao Yongxin; Gu Dongyu; Ayupbek Amatjan; Huang Yun; Dou Jun; Ito Yoichiro; Zhang Tianyou; Aisa Haji Akber
2010
Source PublicationJOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
ISSN1082-6076
Volume33Issue:16Pages:1502-1515
Abstract

An effective high-speed countercurrent chromatography (HSCCC) method was established for further separation and purification of four minor flavonols in addition to five major flavonols which were reported by our previous study from extracts of Flos gossypii. HSCCC was performed with three two-phase solvent systems composed of n-hexane-ethyl acetate-methanol-water (7.5:15:6:7, v/v), (2.5:15:2:7, v/v), and (0:1:0:1, v/v). The separation was repeated three times, and 3.8mg of 8-methoxyl-kaempferol-7-O-β-D-rhamnoside (HPLC purity 98.27%), 6.7mg of astragalin (HPLC purity 94.18%), 3.3mg of 4'-methoxyl-quercetin-7-O- β-D-glucoside (HPLC purity 94.30%), and 8.2mg of hyperoside (HPLC purity 93.48%) were separated from 150mg of the crude sample. The chemical structures of the flavonols were confirmed by MS, 1H NMR, and 13C NMR. Meanwhile, the results indicated that the target compound with smaller K value (<0.5) can be separated by increasing column length of HSCCC. And, four separation rules of flavonols were established according to the present study and references were summarized, which can be used as a useful guide for separation of flavonols by HSCCC.

KeywordFlavonol Flos Gossypii Hsccc Separation Rules Of Flavonols
Subject AreaBiochemistry & Molecular Biology ; Chemistry
DOI10.1080/10826076.2010.489000
Indexed BySCI
WOS IDWOS:000282581800002
Citation statistics
Cited Times:10[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.xjipc.cas.cn/handle/365002/1786
Collection省部共建新疆特有药用资源利用重点实验室
资源化学研究室
Affiliation1.Biochem & Biophys Ctr, Bioseparat Technol Lab, NHLBI, NIH, Bethesda, MD 20892 USA
2.Chinese Acad Sci, Xinjiang Key Lab Plant Resources & Nat Prod Chem, Xinjiang Tech Inst Phys & Chem, Urumqi, Peoples R China
3.Chinese Acad Sci, Key Lab Chem Plant Resources Arid Area, Xinjiang Tech Inst Phys & Chem, Urumqi, Peoples R China
4.Beijing Inst New Technol Applicat, Beijing, Peoples R China
Recommended Citation
GB/T 7714
Yang Yi,Zhao Yongxin,Gu Dongyu,et al. SEPARATION OF THE MINOR FLAVONOLS FROM FLOS GOSSYPII BY HIGH-SPEED COUNTERCURRENT CHROMATOGRAPHY[J]. JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,2010,33(16):1502-1515.
APA Yang Yi.,Zhao Yongxin.,Gu Dongyu.,Ayupbek Amatjan.,Huang Yun.,...&Aisa Haji Akber.(2010).SEPARATION OF THE MINOR FLAVONOLS FROM FLOS GOSSYPII BY HIGH-SPEED COUNTERCURRENT CHROMATOGRAPHY.JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,33(16),1502-1515.
MLA Yang Yi,et al."SEPARATION OF THE MINOR FLAVONOLS FROM FLOS GOSSYPII BY HIGH-SPEED COUNTERCURRENT CHROMATOGRAPHY".JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES 33.16(2010):1502-1515.
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