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学科主题: Biochemistry & Molecular Biology ; Chemistry
题名: SEPARATION OF THE MINOR FLAVONOLS FROM FLOS GOSSYPII BY HIGH-SPEED COUNTERCURRENT CHROMATOGRAPHY
作者: Yang Yi; Zhao Yongxin; Gu Dongyu; Ayupbek Amatjan; Huang Yun; Dou Jun; Ito Yoichiro; Zhang Tianyou; Aisa Haji Akber
关键词: flavonol ; Flos gossypii ; HSCCC ; separation rules of flavonols
刊名: JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
发表日期: 2010
DOI: 10.1080/10826076.2010.489000
卷: 33, 期:16, 页:1502-1515
收录类别: SCI
资助者: Chinese Academy of Sciences Chinese Academy of Sciences National Natural Science Foundation of China
摘要: An effective high-speed countercurrent chromatography (HSCCC) method was established for further separation and purification of four minor flavonols in addition to five major flavonols which were reported by our previous study from extracts of Flos gossypii. HSCCC was performed with three two-phase solvent systems composed of n-hexane-ethyl acetate-methanol-water (7.5:15:6:7, v/v), (2.5:15:2:7, v/v), and (0:1:0:1, v/v). The separation was repeated three times, and 3.8mg of 8-methoxyl-kaempferol-7-O-β-D-rhamnoside (HPLC purity 98.27%), 6.7mg of astragalin (HPLC purity 94.18%), 3.3mg of 4'-methoxyl-quercetin-7-O- β-D-glucoside (HPLC purity 94.30%), and 8.2mg of hyperoside (HPLC purity 93.48%) were separated from 150mg of the crude sample. The chemical structures of the flavonols were confirmed by MS, 1H NMR, and 13C NMR. Meanwhile, the results indicated that the target compound with smaller K value (<0.5) can be separated by increasing column length of HSCCC. And, four separation rules of flavonols were established according to the present study and references were summarized, which can be used as a useful guide for separation of flavonols by HSCCC.
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内容类型: 期刊论文
URI标识: http://ir.xjipc.cas.cn/handle/365002/1786
Appears in Collections:新疆维吾尔自治区植物资源化学重点实验室 _期刊论文

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作者单位: Biochem & Biophys Ctr, Bioseparat Technol Lab, NHLBI, NIH, Bethesda, MD 20892 USA;Chinese Acad Sci, Xinjiang Key Lab Plant Resources & Nat Prod Chem, Xinjiang Tech Inst Phys & Chem, Urumqi, Peoples R China;Chinese Acad Sci, Key Lab Chem Plant Resources Arid Area, Xinjiang Tech Inst Phys & Chem, Urumqi, Peoples R China;Beijing Inst New Technol Applicat, Beijing, Peoples R China

Recommended Citation:
Yang Yi,Zhao Yongxin,Gu Dongyu,et al. SEPARATION OF THE MINOR FLAVONOLS FROM FLOS GOSSYPII BY HIGH-SPEED COUNTERCURRENT CHROMATOGRAPHY[J]. JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,2010,33(16):1502-1515.
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