NOVEL DESIGNS FOR CENTRIFUGAL COUNTERCURRENT CHROMATOGRAPHY: V. COMPARATIVE STUDIES ON PERFORMANCE OF VARIOUS COLUMN CONFIGURATIONS
Yang Yi; Gu Dongyu; Aisa Haji Akber; Ito Yoichiro
2010
发表期刊Journal of Liquid Chromatography and Related Technologies
ISSN10826076
卷号33期号:16页码:1542-1549
摘要The conventional toroidal coil in centrifugal countercurrent chromatography has a low level of stationary phase retention, since a half of each helical turn is entirely occupied by the mobile phase. In order to cope with this problem, several new column designs including zigzag, saw-tooth, and figure-8 patterns have been introduced, and their performance was compared in terms of retention of the stationary phase (Sf), peak resolution (Rs), theoretical plate number (N), and column pressures. Overall results of experiments indicate that the figure-8 column yields the highest Rs when the lower phase is used as the mobile phase. Since the column pressure of all these new columns are much lower than that in the traditional toroidal coil column, the separation efficiency can be improved using a long separation column without a risk of column damage by high back pressure. Copyright © Taylor & Francis Group, LLC.
关键词Centrifugal Countercurrent Chromatography Dipeptide Dnp-amino Acid Figure-8 Column Peak Resolution Saw-tooth Column Stationary Phase Retention Toroidal Coiled Column Zigzag Column
学科领域Biochemistry & Molecular Biology ; Chemistry
DOI10.1080/10826076.2010.503780
收录类别SCI
WOS记录号WOS:000282581800005
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文献类型期刊论文
条目标识符http://ir.xjipc.cas.cn/handle/365002/1785
专题省部共建新疆特有药用资源利用重点实验室
作者单位Biochem & Biophys Ctr, Bioseparat Technol Lab, NHLBI, NIH, Bethesda, MD 20892 USA;Chinese Acad Sci, Xinjiang Key Lab Plant Resources & Nat Prod Chem, Xinjiang Tech Inst Phys & Chem, Urumqi, Peoples R China
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Yang Yi,Gu Dongyu,Aisa Haji Akber,et al. NOVEL DESIGNS FOR CENTRIFUGAL COUNTERCURRENT CHROMATOGRAPHY: V. COMPARATIVE STUDIES ON PERFORMANCE OF VARIOUS COLUMN CONFIGURATIONS[J]. Journal of Liquid Chromatography and Related Technologies,2010,33(16):1542-1549.
APA Yang Yi,Gu Dongyu,Aisa Haji Akber,&Ito Yoichiro.(2010).NOVEL DESIGNS FOR CENTRIFUGAL COUNTERCURRENT CHROMATOGRAPHY: V. COMPARATIVE STUDIES ON PERFORMANCE OF VARIOUS COLUMN CONFIGURATIONS.Journal of Liquid Chromatography and Related Technologies,33(16),1542-1549.
MLA Yang Yi,et al."NOVEL DESIGNS FOR CENTRIFUGAL COUNTERCURRENT CHROMATOGRAPHY: V. COMPARATIVE STUDIES ON PERFORMANCE OF VARIOUS COLUMN CONFIGURATIONS".Journal of Liquid Chromatography and Related Technologies 33.16(2010):1542-1549.
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