|NOVEL DESIGNS FOR CENTRIFUGAL COUNTERCURRENT CHROMATOGRAPHY: V. COMPARATIVE STUDIES ON PERFORMANCE OF VARIOUS COLUMN CONFIGURATIONS|
|Yang Yi; Gu Dongyu; Aisa Haji Akber; Ito Yoichiro
|Source Publication||Journal of Liquid Chromatography and Related Technologies
|Abstract||The conventional toroidal coil in centrifugal countercurrent chromatography has a low level of stationary phase retention, since a half of each helical turn is entirely occupied by the mobile phase. In order to cope with this problem, several new column designs including zigzag, saw-tooth, and figure-8 patterns have been introduced, and their performance was compared in terms of retention of the stationary phase (Sf), peak resolution (Rs), theoretical plate number (N), and column pressures. Overall results of experiments indicate that the figure-8 column yields the highest Rs when the lower phase is used as the mobile phase. Since the column pressure of all these new columns are much lower than that in the traditional toroidal coil column, the separation efficiency can be improved using a long separation column without a risk of column damage by high back pressure. Copyright © Taylor & Francis Group, LLC.|
|Keyword||Centrifugal Countercurrent Chromatography
Stationary Phase Retention
Toroidal Coiled Column
|Subject Area||Biochemistry & Molecular Biology
|Affiliation||Biochem & Biophys Ctr, Bioseparat Technol Lab, NHLBI, NIH, Bethesda, MD 20892 USA;Chinese Acad Sci, Xinjiang Key Lab Plant Resources & Nat Prod Chem, Xinjiang Tech Inst Phys & Chem, Urumqi, Peoples R China|
Yang Yi,Gu Dongyu,Aisa Haji Akber,et al. NOVEL DESIGNS FOR CENTRIFUGAL COUNTERCURRENT CHROMATOGRAPHY: V. COMPARATIVE STUDIES ON PERFORMANCE OF VARIOUS COLUMN CONFIGURATIONS[J]. Journal of Liquid Chromatography and Related Technologies,2010,33(16):1542-1549.
Yang Yi,Gu Dongyu,Aisa Haji Akber,&Ito Yoichiro.(2010).NOVEL DESIGNS FOR CENTRIFUGAL COUNTERCURRENT CHROMATOGRAPHY: V. COMPARATIVE STUDIES ON PERFORMANCE OF VARIOUS COLUMN CONFIGURATIONS.Journal of Liquid Chromatography and Related Technologies,33(16),1542-1549.
Yang Yi,et al."NOVEL DESIGNS FOR CENTRIFUGAL COUNTERCURRENT CHROMATOGRAPHY: V. COMPARATIVE STUDIES ON PERFORMANCE OF VARIOUS COLUMN CONFIGURATIONS".Journal of Liquid Chromatography and Related Technologies 33.16(2010):1542-1549.
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