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学科主题: Biochemistry & Molecular Biology ; Chemistry
题名: NOVEL DESIGNS FOR CENTRIFUGAL COUNTERCURRENT CHROMATOGRAPHY: V. COMPARATIVE STUDIES ON PERFORMANCE OF VARIOUS COLUMN CONFIGURATIONS
作者: Yang Yi; Gu Dongyu; Aisa Haji Akber; Ito Yoichiro
关键词: centrifugal countercurrent chromatography ; dipeptide ; DNP-amino acid ; figure-8 column ; peak resolution ; saw-tooth column ; stationary phase retention ; toroidal coiled column ; zigzag column
刊名: Journal of Liquid Chromatography and Related Technologies
发表日期: 2010
DOI: 10.1080/10826076.2010.503780
卷: 33, 期:16, 页:1542-1549
收录类别: SCI
摘要: The conventional toroidal coil in centrifugal countercurrent chromatography has a low level of stationary phase retention, since a half of each helical turn is entirely occupied by the mobile phase. In order to cope with this problem, several new column designs including zigzag, saw-tooth, and figure-8 patterns have been introduced, and their performance was compared in terms of retention of the stationary phase (Sf), peak resolution (Rs), theoretical plate number (N), and column pressures. Overall results of experiments indicate that the figure-8 column yields the highest Rs when the lower phase is used as the mobile phase. Since the column pressure of all these new columns are much lower than that in the traditional toroidal coil column, the separation efficiency can be improved using a long separation column without a risk of column damage by high back pressure. Copyright © Taylor & Francis Group, LLC.
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内容类型: 期刊论文
URI标识: http://ir.xjipc.cas.cn/handle/365002/1785
Appears in Collections:新疆维吾尔自治区植物资源化学重点实验室 _期刊论文

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作者单位: Biochem & Biophys Ctr, Bioseparat Technol Lab, NHLBI, NIH, Bethesda, MD 20892 USA;Chinese Acad Sci, Xinjiang Key Lab Plant Resources & Nat Prod Chem, Xinjiang Tech Inst Phys & Chem, Urumqi, Peoples R China

Recommended Citation:
Yang Yi,Gu Dongyu,Aisa Haji Akber,et al. NOVEL DESIGNS FOR CENTRIFUGAL COUNTERCURRENT CHROMATOGRAPHY: V. COMPARATIVE STUDIES ON PERFORMANCE OF VARIOUS COLUMN CONFIGURATIONS[J]. Journal of Liquid Chromatography and Related Technologies,2010,33(16):1542-1549.
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文件名: novel designs for centrifugal countercurrent chromatography v comparative studies on performance of various column configurations.pdf
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