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学科主题: Biochemistry & Molecular Biology ; Chemistry
题名: Evaluation of the effect of column orientation in type-I high-speed counter-current chromatography
作者: Yang Yi; Gu Dongyu; Aisa Haji Akber; Ito Yoichiro
关键词: Type-I counter-current chromatography ; Coil planet centrifuge ; The angle against the rotating centrifugal ; force field ; Retention of the stationary phase ; Resolution ; Dipeptide ; DNP-amino acid
刊名: JOURNAL OF CHROMATOGRAPHY A
发表日期: 2010
DOI: 10.1016/j.chroma.2010.03.004
卷: 1217, 期:18, 页:3167-3170
收录类别: SCI
摘要: The performance of type-I high-speed counter-current chromatography was evaluated by changing the column inclination against the rotating centrifugal force field. The separations were performed with two different solvent systems composed of 1-butanol-acetic acid-water (4.75:0.25:5, v/v) (BAW) and hexane-ethyl acetate-methanol-0.1. M HCl (1:1:1:1, v/v) (HEMW) using dipeptides and DNP-amino acid as test samples, respectively. A set of short coiled columns connected in series is mounted around the holder hub in two different ways: in the parallel orientation, all column units are arranged in parallel to each other and mounted on the holder at various angles against the horizontal plane. In the zigzag configuration, the neighboring units of the same column are mounted symmetrically forming various angles apart. In the parallel configuration, for both the BAW and HEMW systems, Sf (the retention of stationary phase) first increased as the column angle decreased from 90° to 60° and then decreased, as the column angle further decreased from 60° to 0°, while Rs (peak resolution) continually declined over the entire column angle range from 90° to 0°. But, for both solvent systems, with the zigzag configuration, retention of stationary phase and resolution both decreased as the column angle decreased from 90° to 0°. In general, Sf and Rs for separation of dipeptides in the BAW system, from 90° to 15°, is better for the parallel orientation than for the zigzag configuration. However, at 0°, Sf and Rs are better for the zigzag orientation. In the DNP-amino acid separation with the HEMW system, retention of the stationary phase and Rs for the parallel orientation is better than that for the zigzag orientation from 90° to 30°, whereas from 30° to 0° the results are opposite. Over all results of our studies revealed that the formally used column orientation [5] at 90° inclination yields the highest peak resolution in both solvent systems.
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内容类型: 期刊论文
URI标识: http://ir.xjipc.cas.cn/handle/365002/1784
Appears in Collections:新疆维吾尔自治区植物资源化学重点实验室 _期刊论文

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作者单位: NHLBI, Bioseparat Technol Lab, Biochem & Biophys Ctr, NIH, Bethesda, MD 20892 USA;Chinese Acad Sci, Key Lab Chem Plant Resources Arid Reg, Xinjiang Tech Inst Phys & Chem, Urumqi 830011, Peoples R China

Recommended Citation:
Yang Yi,Gu Dongyu,Aisa Haji Akber,et al. Evaluation of the effect of column orientation in type-I high-speed counter-current chromatography[J]. JOURNAL OF CHROMATOGRAPHY A,2010,1217(18):3167-3170.
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