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题名: preparative isolation and purification of isobenzofuranone derivatives and saponins from seeds of nigella glandulifera freyn by high-speed counter-current chromatography combined with gel filtration
作者: Xin Xuelei; Yang Yi; Zhong Jie; Aisa Haji Akber; Wang Hanqing
通讯作者: Aisa, HA
关键词: High-speed counter-current chromatography ; Nigella glandulifera Freyn ; Seeds ; Isobenzofuranone ; Salfredin B11
刊名: Journal of Chromatography A
发表日期: 2009
DOI: 10.1016/j.chroma.2009.03.050
卷: 1216, 期:19, 页:4258-4262
收录类别: EI
摘要: Although the medicinal plant and food Nigella glandulifera Freyn has been researched for decades, isobenzofuranones have never been isolated before. Two isobenzofuranone derivatives and two saponins were successfully separated and purified from seeds of N. glandulifera Freyn by high-speed counter-current chromatography (HSCCC) with the optimized two-phase solvent system, n-hexane-ethyl acetate-methanol-water (7:3:5:5, v/v). Salfredin B11 (22.1 mg, HPLC purity 95.3%), 5, 7-dihydroxy-6-(3-methybut-2-enyl) isobenzofuran-1(3H)-one (18.9 mg, HPLC purity 97.3%) and crude sample 2 (555 mg) were separated from 600 mg of ethyl acetate extract of N. glandulifera Freyn. Following a cleaning-up step by chromatography on Sephadex LH-20, hederagenin (12 mg) and 3-O-[β-d-xylopyranosyl-(1 &rarr 3)-α-l-rhamnopyranosyl-(1 &rarr 2)-α-l-arabinopyranosyl]-hederagenin (45 mg) were separated from sample 2. All of the fractions before peak II were collected and subjected to a Sephadex LH-20 column and eluted by methanol, two of triterpene saponins (12 mg of hederagenin and 45 mg of 3-O-[β-d-xylopyranosyl-(1 &rarr 3)-α-l-rhamnopyranosyl-(1 &rarr 2)-α-l-arabinopyranosyl]-hederagenin) were isolated. The structures of peak fractions were identified by IR, electron ionization MS, 1H NMR and 13C NMR. 5, 7-Dihydroxy-6-(3-methybut-2-enyl) isobenzofuran-1(3H)-one was isolated for the first time from higher plant and salfredin B11 was isolated for the first time in this plant.
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内容类型: 期刊论文
URI标识: http://ir.xjipc.cas.cn/handle/365002/1783
Appears in Collections:新疆维吾尔自治区植物资源化学重点实验室 _期刊论文

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作者单位: Chinese Acad Sci, Xinjiang Tech Inst Phys & Chem, Xinjiang Key Lab Plant Resources & Nat Prod Chem, Urumqi 830011, Peoples R China;Chinese Acad Sci, Lanzhou Inst Chem Phys, Lanzhou 730000, Peoples R China;Chinese Acad Sci, Xinjiang Tech Inst Phys & Chem, Key Lab Chem Plant Resources Arid Area, Urumqi 830011, Peoples R China

Recommended Citation:
Xin Xuelei,Yang Yi,Zhong Jie,et al. preparative isolation and purification of isobenzofuranone derivatives and saponins from seeds of nigella glandulifera freyn by high-speed counter-current chromatography combined with gel filtration[J]. Journal of Chromatography A,2009,1216(19):4258-4262.
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文件名: preparative isolation and purification of isobenzofuranone derivatives and saponins from seeds of nigella glandulifera freyn by high-speed counter-current chromatography combined with gel filtration.pdf
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