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NOVEL DESIGN FOR CENTRIFUGAL COUNTERCURRENT CHROMATOGRAPHY: IV. FIGURE-8 COLUMN
Gu Dongyu; Yang Yi; Eng Jennifer D.; Aisa Haji Akber; Ito Yoichiro
2010
发表期刊JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
ISSN10826076
卷号33期号:4页码:572-584
摘要The toroidal columns using an equilateral triangular core, zigzag, and saw patterns has improved step by step both for retention of the stationary phase and peak resolution of the conventional toroidal coil in centrifugal countercurrent chromatography. To further improve the retention of stationary phase and peak resolution, a novel figure 8 toroidal coil was designed and the performance of the system was evaluated at various flow rates. In the figure-8 column with a capacity of 3mL, the peak resolution of dipeptides (Val-Tyr and Trp-Tyr) was 1.63, and that of DNP-amino acids (DNP-DL-glu, DNP - ala, DNP-L-ala) were 1.92 and 1.85, respectively. The results indicated that retention of stationary phase and peak resolution were improved at lower flow rates. The Rs with lower mobile phase can be further increased by modifying the tubing to form a flat twisted configuration. With a decreased column length with a capacity of about 0.1mL, resolution of the figure-8 column was 0.71 with about 40% retention of stationary phase at a flow rate of 0.005mL/min.
关键词Countercurrent Chromatography Dipeptide Dnp-amino Acid Figure-8 Column Peak Resolution Retention Of The Stationary Phase
学科领域Biochemistry & Molecular Biology ; Chemistry
DOI10.1080/10826070903574675
收录类别SCI
WOS记录号WOS:000274536100014
引用统计
被引频次:9[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.xjipc.cas.cn/handle/365002/1779
专题资源化学研究室
作者单位NHLBI, NIH, Bethesda, MD 20892 USA;Chinese Acad Sci, Xinjiang Tech Inst Phys & Chem, Urumqi, Peoples R China;Chinese Acad Sci, Grad Univ, Beijing, Peoples R China
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GB/T 7714
Gu Dongyu,Yang Yi,Eng Jennifer D.,et al. NOVEL DESIGN FOR CENTRIFUGAL COUNTERCURRENT CHROMATOGRAPHY: IV. FIGURE-8 COLUMN[J]. JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,2010,33(4):572-584.
APA Gu Dongyu,Yang Yi,Eng Jennifer D.,Aisa Haji Akber,&Ito Yoichiro.(2010).NOVEL DESIGN FOR CENTRIFUGAL COUNTERCURRENT CHROMATOGRAPHY: IV. FIGURE-8 COLUMN.JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,33(4),572-584.
MLA Gu Dongyu,et al."NOVEL DESIGN FOR CENTRIFUGAL COUNTERCURRENT CHROMATOGRAPHY: IV. FIGURE-8 COLUMN".JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES 33.4(2010):572-584.
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