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Comparative Study on Separation and Purification of Isoflavones from the Seeds and Sprouts of Chickpea by High-Speed Countercurrent Chromatography
Lv Qiaoying; Yang Yi; Zhao Yongxin; Gu Dongyu; He Dajun; Yili Abulimiti; Ma Qingling; Cheng Zhen; Gao Yanhua; Aisa Haji Akber; Ito Yoichiro
2009
Source PublicationJOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
ISSN10826076
Volume32Issue:19Pages:2879-2892
AbstractChickpea is known as a plant that is rich in protein, carbohydrates, and nutrition, and its seeds and sprouts have been processed into various health foods. In the present study, four isoflavones were purified from the seeds and sprouts of chickpea by high-speed countercurrent chromatography (HSCCC) using two biphasic solvent systems composed of n-hexane-ethyl acetate-methanol-water (5:5:5:5, v/v) and ethyl acetate-water (1:1, v/v). The results indicated that 14.2mg of formononetin, 15.7mg of biochanin A, 9.1mg of ononin, 11.3mg of biochanin A-7-O-β- D-glucoside were obtained from 150mg of sprout extracts with the purity of 92.26%, 95.86%, 95.32%, and 96.56%, respectively. Compared with the sprouts, separation of seed extracts yielded less amounts of biochanin A-7-O-β - D-glucoside and biochanin A with lower purity. The results indicate that four main isoflavones in chickpea, i.e., isoflavones, formononetin, biochanin A, ononin, and biochanin A-7-O-β- D-glucoside, are substantially increased by biosynthesis during the seed germination. © Taylor & Francis Group, LLC.
KeywordBiosynthesis Chickpea High Speed Countercurrent Chromatography Isoflavone Seed Sprout
Subject AreaBiochemistry & Molecular Biology ; Chemistry
DOI10.1080/10826070903297277
WOS IDWOS:000270812700009
Citation statistics
Cited Times:14[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.xjipc.cas.cn/handle/365002/1778
Collection省部共建新疆特有药用资源利用重点实验室
AffiliationNHLBI, Bioseparat Technol Lab, Biochem & Biophys Ctr, NIH, Bethesda, MD 20892 USA;Chinese Acad Sci, Xinjiang Tech Inst Phys & Chem, Xinjiang Key Lab Plant Resources & Nat Prod Chem, Urumqi, Peoples R China;Chinese Acad Sci, Grad Univ, Beijing, Peoples R China;Union Labs Bioact Ingredients Edible Plants Cent, Urumqi, Peoples R China
Recommended Citation
GB/T 7714
Lv Qiaoying,Yang Yi,Zhao Yongxin,et al. Comparative Study on Separation and Purification of Isoflavones from the Seeds and Sprouts of Chickpea by High-Speed Countercurrent Chromatography[J]. JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,2009,32(19):2879-2892.
APA Lv Qiaoying.,Yang Yi.,Zhao Yongxin.,Gu Dongyu.,He Dajun.,...&Ito Yoichiro.(2009).Comparative Study on Separation and Purification of Isoflavones from the Seeds and Sprouts of Chickpea by High-Speed Countercurrent Chromatography.JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,32(19),2879-2892.
MLA Lv Qiaoying,et al."Comparative Study on Separation and Purification of Isoflavones from the Seeds and Sprouts of Chickpea by High-Speed Countercurrent Chromatography".JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES 32.19(2009):2879-2892.
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