中国科学院新疆理化技术研究所机构知识库
Advanced  
XJIPC OpenIR  > 资源化学研究室  > 期刊论文
学科主题: Biochemistry & Molecular Biology ; Chemistry
题名: Evaluation on the performance of four different column models mounted on the compact type-I coil planet centrifuge
作者: Yang Yi; Gu Dongyu; Aisa Haji Akber; Ito Yoichiro
关键词: Compact type-I coil planet centrifuge ; Counter current chromatography ; Mounted column models ; DNP-amino acids ; Dipeptides ; Retention of the stationary phase ; Resolution
刊名: Journal of Chromatography A
发表日期: 2010
DOI: 10.1016/j.chroma.2010.10.012
卷: 1217, 期:48, 页:7612-7615
资助者: Intramural NIH HHS
摘要: Optimal positions of coiled separation columns on the type-I centrifuge were determined for four typical two-phase solvent systems to obtain the best separation efficiency (resolution and retention of stationary phase) for each with a suitable set of test samples. A set of short coiled columns is connected in series and mounted around the holder hub in four different ways: (model A) the tail of one unit with left-handedness was connected to the head of the next unit with right-handedness (TL-HR); (model B) the tail of one unit with left-handedness was connected to the tail of the next unit with right-handedness (TL-TR); (model C) the tail of one unit with left-handedness was connected to the tail of the next unit with left-handedness (TL-TL); (model D) the tail of one unit with left-handedness was connected to the head of the next unit with left-handedness (TL-HL). The results indicated that the performance of model D was the best among the four models. High revolution speed (800. rpm) is favorable to separation using the moderately hydrophobic solvent system of hexane-ethyl acetate-methanol-0.1. M HCl (1:1:1:1, v/v) (HEMW), while lower revolution speed (600. rpm) is beneficial to the separation with polar solvent system of 1-butanol-acetic acid-water (19:1:20, v/v) (BAW).
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.xjipc.cas.cn/handle/365002/1776
Appears in Collections:资源化学研究室_期刊论文

Files in This Item:
File Name/ File Size Content Type Version Access License
evaluation on the performance of four different column models mounted on the compact type-i coil planet centrifuge.pdf(401KB)期刊论文作者接受稿开放获取View 联系获取全文

作者单位: NHLBI, Bioseparat Technol Lab, Biochem & Biophys Ctr, NIH, Bethesda, MD 20892 USA;Chinese Acad Sci, Key Lab Xinjiang Indigenous Med Plants Resource U, Xinjiang Tech Inst Phys & Chem, Urumqi 830011, Peoples R China

Recommended Citation:
Yang Yi,Gu Dongyu,Aisa Haji Akber,et al. Evaluation on the performance of four different column models mounted on the compact type-I coil planet centrifuge[J]. Journal of Chromatography A,2010,1217(48):7612-7615.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Yang Yi]'s Articles
[Gu Dongyu]'s Articles
[Aisa Haji Akber]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Yang Yi]‘s Articles
[Gu Dongyu]‘s Articles
[Aisa Haji Akber]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
文件名: evaluation on the performance of four different column models mounted on the compact type-i coil planet centrifuge.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Powered by CSpace