NOVEL DESIGN FOR CENTRIFUGAL COUNTER-CURRENT CHROMATOGRAPHY: III. SAW TOOTH COLUMN
Yang Yi; Aisa Haji Akber; Ito Yoichiro
2010
发表期刊JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
ISSN10826076
卷号33期号:6页码:846-858
摘要The toroidal coil using an equilateral triangular core and zigzag pattern column have improved both retention of the stationary phase and peak resolution of the conventional toroidal coil in centrifugal counter-current chromatography. To further improve the retention of stationary phase and peak resolution, a novel saw tooth column was designed and the performance of the system was evaluated at various flow rates. The results indicated that both retention of the stationary phase and peak resolution were improved as the flow rate was decreased and at a flow rate of 0.005mL/min the resolution is remarkably increased. Modification of the tubing called flat-twisted tubing further improved the peak resolution without increasing the column pressure. With decreased column length at a capacity of about 0.2mL, resolution of the saw tooth column was 1.02.
关键词Counter-current Chromatography Dipeptide Dnp-amino Acid Resolution Retention Of The Stationary Phase Saw Tooth Column
学科领域Biochemistry & Molecular Biology ; Chemistry
DOI10.1080/10826071003684547
收录类别SCI
WOS记录号WOS:000277574500012
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文献类型期刊论文
条目标识符http://ir.xjipc.cas.cn/handle/365002/1775
专题省部共建新疆特有药用资源利用重点实验室
作者单位NHLBI, Bioseparat Technol Lab, Biochem & Biophys Ctr, NIH, Bethesda, MD 20892 USA;Chinese Acad Sci, Xinjiang Key Lab Plant Resources & Nat Prod Chem, Xinjiang Tech Inst Phys & Chem, Urumqi, Peoples R China;Chinese Acad Sci, Grad Univ, Beijing, Peoples R China
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Yang Yi,Aisa Haji Akber,Ito Yoichiro. NOVEL DESIGN FOR CENTRIFUGAL COUNTER-CURRENT CHROMATOGRAPHY: III. SAW TOOTH COLUMN[J]. JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,2010,33(6):846-858.
APA Yang Yi,Aisa Haji Akber,&Ito Yoichiro.(2010).NOVEL DESIGN FOR CENTRIFUGAL COUNTER-CURRENT CHROMATOGRAPHY: III. SAW TOOTH COLUMN.JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,33(6),846-858.
MLA Yang Yi,et al."NOVEL DESIGN FOR CENTRIFUGAL COUNTER-CURRENT CHROMATOGRAPHY: III. SAW TOOTH COLUMN".JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES 33.6(2010):846-858.
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