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野生花卉厚叶岩白菜组织培养及再生体系的建立
Alternative Titletissue culture of wild flower bergenia crassifolia and establishment of its regeneration system
刘敏; 郝秀英; 徐琴; 波拉提; 康喜亮; 王晓军
2009
Source Publication安徽农业科学
ISSN0517-6611
Volume37Issue:8Pages:3455-3456,3462
Abstract[目的]建立厚叶岩白菜的完整再生体系,并为研究其遗传转化奠定基础。[方法]以野生厚叶岩白菜的叶片为外植体,通过筛选培养基与激素配比,初步建立其离体培养再生体系。[结果]厚叶岩白菜愈伤组织的最佳诱导培养基为:改良MS+NAA 0.50 mg/L+6-BA 0.50 mg/L+水解酪蛋白100.00 mg/L+0.2%PVP;分化培养基为:改良MS+NAA 0.50 mg/L+IBA 0.10 mg/L+6-BA0.50 mg/L;生根培养基为:1/2 MS+IBA 0.50 mg/L+NAA 0.01 mg/L。15 d后移植幼苗的叶片由嫩绿变成深绿,30 d后移植幼苗长出新叶,叶片中心偏红,成活率达到95%,且幼苗生长健壮。[结论]通过对培养基组分、激素配比及环境因子的筛选,为野生厚叶岩白菜初步建立了一个实用的离体培养再生技术体系。
Keyword野生厚叶岩白菜 叶片外植体 组织培养 愈伤组织 离体再生
DOI10.13989/j.cnki.0517-6611.2009.08.123
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Document Type期刊论文
Identifierhttp://ir.xjipc.cas.cn/handle/365002/1771
Collection资源化学研究室
Affiliation中国科学院新疆理化技术研究所
Recommended Citation
GB/T 7714
刘敏,郝秀英,徐琴,等. 野生花卉厚叶岩白菜组织培养及再生体系的建立[J]. 安徽农业科学,2009,37(8):3455-3456,3462.
APA 刘敏,郝秀英,徐琴,波拉提,康喜亮,&王晓军.(2009).野生花卉厚叶岩白菜组织培养及再生体系的建立.安徽农业科学,37(8),3455-3456,3462.
MLA 刘敏,et al."野生花卉厚叶岩白菜组织培养及再生体系的建立".安徽农业科学 37.8(2009):3455-3456,3462.
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