Rapid Desalting of Recombinant Human Erythropoietin Sample by Microelectro Purification Device for Mass Spectrometric Detection
Pang Nan-Nan; Zhou Yu; Bai Yu; Liao Jie; Liu Hu-Wei
2011
发表期刊CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
ISSN0253-3820
卷号39期号:2页码:231-234
摘要Microelectro purification device (MEPD) constructed by our laboratory could obtain online desalting of recombinant human erythropoietin (rhEPO) in 2 min with high efficiency. Electrospray mass spectrometry with positive ion mode was used for the detection. Compared with traditional methods, such as gradient elution by C(18)-reversed phase liquid chromatography (150 mm X4. 6 mm, 5 mu m) as well as ultra-filtration, MEPD could further enhance the signal to noise ratio of rhEPO and the detection sensitivity. It could also shorten the analysis time dramatically. This simple and rapid method could be a promising pretreatment candidate for the rhEPO analysis of mass spectrometry.
关键词Liquid Chromatography Mass Spectrometry Microelectro Purification Device Ultra-filtration Recombinant Human Erythropoietin
学科领域Chemistry
DOI10.3724/SP.J.1096.2011.00231
收录类别SCI
WOS记录号WOS:000290240800015
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文献类型期刊论文
条目标识符http://ir.xjipc.cas.cn/handle/365002/1672
专题省部共建新疆特有药用资源利用重点实验室
作者单位Peking Univ, Coll Chem & Chem Engn, Beijing Natl Lab Mol Sci, Minist Educ,Inst Analyt Chem, Beijing 100871, Peoples R China;Chinese Acad Sci, Key Lab Plant Resources & Chem Arid Zone, Xinjiang Tech Inst Phys & Chem, Urumqi 830011, Peoples R China;Gen Hosp Chinese Peoples Liberat Army, Med Expt & Anal Ctr, Beijing 100853, Peoples R China
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Pang Nan-Nan,Zhou Yu,Bai Yu,et al. Rapid Desalting of Recombinant Human Erythropoietin Sample by Microelectro Purification Device for Mass Spectrometric Detection[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2011,39(2):231-234.
APA Pang Nan-Nan,Zhou Yu,Bai Yu,Liao Jie,&Liu Hu-Wei.(2011).Rapid Desalting of Recombinant Human Erythropoietin Sample by Microelectro Purification Device for Mass Spectrometric Detection.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,39(2),231-234.
MLA Pang Nan-Nan,et al."Rapid Desalting of Recombinant Human Erythropoietin Sample by Microelectro Purification Device for Mass Spectrometric Detection".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 39.2(2011):231-234.
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