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学科主题: Chemistry
题名: Rapid Desalting of Recombinant Human Erythropoietin Sample by Microelectro Purification Device for Mass Spectrometric Detection
作者: Pang Nan-Nan; Zhou Yu; Bai Yu; Liao Jie; Liu Hu-Wei
关键词: Liquid chromatography ; Mass spectrometry ; Microelectro purification device ; Ultra-filtration ; Recombinant human erythropoietin
刊名: CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
发表日期: 2011
DOI: 10.3724/SP.J.1096.2011.00231
卷: 39, 期:2, 页:231-234
收录类别: SCI
英文摘要: Microelectro purification device (MEPD) constructed by our laboratory could obtain online desalting of recombinant human erythropoietin (rhEPO) in 2 min with high efficiency. Electrospray mass spectrometry with positive ion mode was used for the detection. Compared with traditional methods, such as gradient elution by C(18)-reversed phase liquid chromatography (150 mm X4. 6 mm, 5 mu m) as well as ultra-filtration, MEPD could further enhance the signal to noise ratio of rhEPO and the detection sensitivity. It could also shorten the analysis time dramatically. This simple and rapid method could be a promising pretreatment candidate for the rhEPO analysis of mass spectrometry.
WOS记录号: WOS:000290240800015
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内容类型: 期刊论文
URI标识: http://ir.xjipc.cas.cn/handle/365002/1672
Appears in Collections:新疆维吾尔自治区植物资源化学重点实验室 _期刊论文

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作者单位: Peking Univ, Coll Chem & Chem Engn, Beijing Natl Lab Mol Sci, Minist Educ,Inst Analyt Chem, Beijing 100871, Peoples R China;Chinese Acad Sci, Key Lab Plant Resources & Chem Arid Zone, Xinjiang Tech Inst Phys & Chem, Urumqi 830011, Peoples R China;Gen Hosp Chinese Peoples Liberat Army, Med Expt & Anal Ctr, Beijing 100853, Peoples R China

Recommended Citation:
Pang Nan-Nan,Zhou Yu,Bai Yu,et al. Rapid Desalting of Recombinant Human Erythropoietin Sample by Microelectro Purification Device for Mass Spectrometric Detection[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2011,39(2):231-234.
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文件名: rapid desalting of recombinant human erythropoietin sample by microelectro purification device for mass spectrometric detection.pdf
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