XJIPC OpenIR  > 省部共建新疆特有药用资源利用重点实验室
三种维药提取物对蛋白酪氨酸磷酸酶1B抑制作用的研究
Alternative Titleinhibitory effect of extracts from three uyghur medicine on the protein tyrosine phosphatase 1b activity
刘禾培; 窦君; 马纪; 信学雷; 赵海清; 阿吉艾克拜尔·艾萨
2011
Source Publication天然产物研究与开发
ISSN1001-6880
Volume23Issue:1Pages:142-145
Abstract

蛋白酪氨酸磷酸酶1B(PTP1B)是胰岛素增敏的新靶点之一。本文研究了芳香新塔花、沙生蜡菊、驱虫斑鸠菊等3种维药石油醚提取物对PTP1B活性的影响及其对酶的抑制类型。结果表明,所构建的原核表达系统能高表达重组PTP1B(his-PTP1B1-321),分子量为40.8 kDa。3种维药提取物对PTP1B均表现出不同程度的抑制活性,其IC50值分别为20.2μg/mL,12.65μg/mL和9.7μg/mL。芳香新塔花提取物抑制作用类型为非竞争性,沙生蜡菊提取物是竞争性,驱虫斑鸠菊提取物为混合性竞争抑制;抑制常数(Ki)分别为为0.275μg/mL,1.245μg/mL,0.192μg/mL。本研究结果将为维药的进一步开发和利用提供了理论依据。

Other Abstract

Protein tyrosine phosphatase-1B is one of new drug targets against insulin sensitizer. In this study,we investigated the inhibitory effect of petroleumether extracts from 3 Uygur herbs, including Ziziphora Clinopodioides Lam., Helichrysum arenarium(Linn.) Moench, and Ver-nohia anthelmintica(L.) willd,on the PTP1B activity and the mode of inhibition. The results showed that recombinant PTP1B was highly expressed by the prokaryotic expression system and its molecular weigh was 40.8 kDa. Three extracts showed different inhibitory effects on the PTP1B activity, with IC50 values of 20.2 mug/mL,12.65 mug/mL, and 9.7 mug/mL, respectively. The enzyme kinetic studies revealed non-competitive type of inhibition, competitive type of inhibition, and mixed competitive type of inhibition with the inhibitory constants(Ki) of 0.275 mug/mL,1.245 mug/mL, and 0.192 mug/mL, respectively. The study will provide support for further exploration and utilization of Uygur herbs

Keyword维药 蛋白酪氨酸磷酸酯酶1b 抑制作用 原核表达
Subject AreaPlant Sciences ; General & Internal Medicine ; Pharmacology & Pharmacy (Provided By Thomson Reuters)
Indexed ByCSCD
CSCD IDCSCD:4166617
Citation statistics
Document Type期刊论文
Identifierhttp://ir.xjipc.cas.cn/handle/365002/1534
Collection省部共建新疆特有药用资源利用重点实验室
Affiliation新疆大学生命科学与技术学院新疆生物资源基因工程国家重点实验室培育基地;中国科学院新疆理化技术研究所新疆植物资源化学重点实验室
Recommended Citation
GB/T 7714
刘禾培,窦君,马纪,等. 三种维药提取物对蛋白酪氨酸磷酸酶1B抑制作用的研究[J]. 天然产物研究与开发,2011,23(1):142-145.
APA 刘禾培,窦君,马纪,信学雷,赵海清,&阿吉艾克拜尔·艾萨.(2011).三种维药提取物对蛋白酪氨酸磷酸酶1B抑制作用的研究.天然产物研究与开发,23(1),142-145.
MLA 刘禾培,et al."三种维药提取物对蛋白酪氨酸磷酸酶1B抑制作用的研究".天然产物研究与开发 23.1(2011):142-145.
Files in This Item:
File Name/Size DocType Version Access License
三种维药提取物对蛋白酪氨酸磷酸酶1B抑制(269KB)期刊论文作者接受稿开放获取CC BY-NC-SAView Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[刘禾培]'s Articles
[窦君]'s Articles
[马纪]'s Articles
Baidu academic
Similar articles in Baidu academic
[刘禾培]'s Articles
[窦君]'s Articles
[马纪]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[刘禾培]'s Articles
[窦君]'s Articles
[马纪]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: 三种维药提取物对蛋白酪氨酸磷酸酶1B抑制作用的研究.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.