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题名: studies on the effect of column angle in figure-8 centrifugal counter-current chromatography
作者: Yang Yi; Gu Dongyu; Aisa Haji Akber; Ito Yoichiro
关键词: Centrifugal counter-current chromatography ; Figure-8 column ; Angle between column axis and centrifugal force ; Retention of the stationary phase ; Resolution ; Dipeptide ; DNP-amino acid
刊名: Journal of Chromatography A
发表日期: 2011
DOI: 10.1016/j.chroma.2010.11.014
卷: 1218, 期:36, 页:6128-6134
收录类别: SCI
摘要: The performance of the figure-8 column configuration in centrifugal counter-current chromatography was investigated by changing the angle between the column axis (a line through the central post and the peripheral post on which the figure-8 coil is wound) and the centrifugal force. The first series of experiments was performed using a polar two-phase solvent system composed of 1-butanol-acetic acid-water (4:1:5, v/v) to separate two dipeptide samples, Trp-Tyr and Val-Tyr, at a flow rate of 0.05. ml/min at 1000. rpm. When the column angle was changed from 0° (column axis parallel to the centrifugal force) to 45° and 45° to 90° (column axis perpendicular to the centrifugal force), peak resolution (Rs) changed from 1.93 (Sf = 37.8%) to 1.54 (Sf = 30.6%), then to 1.31 (Sf = 40.5%) with the lower mobile phase and from 1.21 (Sf = 38.8%) to 1.10 (Sf = 34.4%), then to 0.99 (Sf = 42.2%) with the upper mobile phase, respectively, where the stationary phase retention, Sf, is given in parentheses. The second series of experiments was similarly performed with a more hydrophobic two-phase solvent system composed of hexane-ethyl acetate-methanol-0.1. M hydrochloric acid (1:1:1:1, v/v) to separate three DNP-amino acids, DNP-glu, DNP-β-ala and DNP-ala, at a flow rate of 0.05. ml/min at 1000. rpm. When the column angle was altered from 0° to 45° and 45° to 90°, Rs changed from 1.77 (1st peak/2nd peak) and 1.52 (2nd peak/3rd peak) (Sf = 27.3%) to 1.24 and 1.02 (Sf = 35.4%), then to 1.69 and 1.49 (Sf = 42.1%) with the lower mobile phase, and from 1.73 and 0.84 (SF = 41.2%) to 1.44 and 0.73 (Sf = 45.6%), then to 1.21 and 0.63 (Sf = 55.6%) with the upper mobile phase, respectively. The performance of figure-8 column at 0° and 90° was also compared at different flow rates. The results show that Rs was increased with decreased flow rate yielding the highest value at the 0° column angle with lower mobile phase. The overall results of our studies indicated that a 0° column angle for the figure-8 column enhances the mixing of two phases in the column to improve peak resolution while decreasing the stationary phase retention by interrupting the laminar flow of the mobile phase.
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内容类型: 期刊论文
URI标识: http://ir.xjipc.cas.cn/handle/365002/1458
Appears in Collections:新疆维吾尔自治区植物资源化学重点实验室 _期刊论文

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作者单位: NHLBI, Bioseparat Technol Lab, Biochem & Biophys Ctr, NIH, Bethesda, MD 20892 USA;Chinese Acad Sci, Xinjiang Tech Inst Phys & Chem, Key Lab Chem Plant Resources Arid Reg, Urumqi 830011, Peoples R China;Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China

Recommended Citation:
Yang Yi,Gu Dongyu,Aisa Haji Akber,et al. studies on the effect of column angle in figure-8 centrifugal counter-current chromatography[J]. Journal of Chromatography A,2011,1218(36):6128-6134.
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