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学科主题: Biochemistry & Molecular Biology ; Chemistry
题名: Evaluation of the performance of protein separation in figure-8 centrifugal counter-current chromatography
作者: Yang Yi; Gu Dongyu; Aisa Haji Akber; Ito Yoichiro
关键词: Centrifugal counter-current chromatography ; Figure-8 column ; Protein separation ; Polymer phase system ; Lysozyme ; Myoglobin ; Hollow fiber membrane dialyzer
刊名: JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
发表日期: 2011
DOI: 10.1016/j.jchromb.2011.10.019
卷: 879, 期:32, 页:3802-3805
摘要: The performance of protein separation using the figure-8 column configuration in centrifugal countercurrent chromatography was investigated under various flow rates and revolution speeds. The separation was performed with a two-phase solvent system composed of polyethylene glycol 1000/potassium phosphate each at 12.5% (w/w) in water and with lysozyme and myoglobin as test samples. In order to improve tracing of the elution curve, a hollow fiber membrane dialyzer was inserted at the inlet of the UV detector. The results showed that the retention of stationary phase (Sf) and resolution (Rs) increased with decreased flow rate and increased revolution speed. The highest Rs of approximately 1 was obtained at a flow rate of 0.01 mL/min under a revolution speed of 1200 rpm with a 3.4 mL capacity column.
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内容类型: 期刊论文
URI标识: http://ir.xjipc.cas.cn/handle/365002/1449
Appears in Collections:新疆维吾尔自治区植物资源化学重点实验室 _期刊论文

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作者单位: NHLBI, Bioseparat Technol Lab, Biochem & Biophys Ctr, NIH, Bethesda, MD 20892 USA;Chinese Acad Sci, Key Lab Chem Plant Resources Arid Reg, Xinjiang Tech Inst Phys & Chem, Urumqi 830011, Peoples R China

Recommended Citation:
Yang Yi,Gu Dongyu,Aisa Haji Akber,et al. Evaluation of the performance of protein separation in figure-8 centrifugal counter-current chromatography[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2011,879(32):3802-3805.
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