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Evaluation of the performance of protein separation in figure-8 centrifugal counter-current chromatography
Yang Yi; Gu Dongyu; Aisa Haji Akber; Ito Yoichiro
2011
Source PublicationJOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
ISSN15700232
Volume879Issue:32Pages:3802-3805
AbstractThe performance of protein separation using the figure-8 column configuration in centrifugal countercurrent chromatography was investigated under various flow rates and revolution speeds. The separation was performed with a two-phase solvent system composed of polyethylene glycol 1000/potassium phosphate each at 12.5% (w/w) in water and with lysozyme and myoglobin as test samples. In order to improve tracing of the elution curve, a hollow fiber membrane dialyzer was inserted at the inlet of the UV detector. The results showed that the retention of stationary phase (Sf) and resolution (Rs) increased with decreased flow rate and increased revolution speed. The highest Rs of approximately 1 was obtained at a flow rate of 0.01 mL/min under a revolution speed of 1200 rpm with a 3.4 mL capacity column.
KeywordCentrifugal Counter-current Chromatography Figure-8 Column Protein Separation Polymer Phase System Lysozyme Myoglobin Hollow Fiber Membrane Dialyzer
Subject AreaBiochemistry & Molecular Biology ; Chemistry
DOI10.1016/j.jchromb.2011.10.019
WOS IDWOS:000298530200004
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Document Type期刊论文
Identifierhttp://ir.xjipc.cas.cn/handle/365002/1449
Collection省部共建新疆特有药用资源利用重点实验室
AffiliationNHLBI, Bioseparat Technol Lab, Biochem & Biophys Ctr, NIH, Bethesda, MD 20892 USA;Chinese Acad Sci, Key Lab Chem Plant Resources Arid Reg, Xinjiang Tech Inst Phys & Chem, Urumqi 830011, Peoples R China
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Yang Yi,Gu Dongyu,Aisa Haji Akber,et al. Evaluation of the performance of protein separation in figure-8 centrifugal counter-current chromatography[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2011,879(32):3802-3805.
APA Yang Yi,Gu Dongyu,Aisa Haji Akber,&Ito Yoichiro.(2011).Evaluation of the performance of protein separation in figure-8 centrifugal counter-current chromatography.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,879(32),3802-3805.
MLA Yang Yi,et al."Evaluation of the performance of protein separation in figure-8 centrifugal counter-current chromatography".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 879.32(2011):3802-3805.
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