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studies on the performance of different coiled column configurations for compact type-i countercurrent chromatography
Yang Yi; Gu Dongyu; Aisa Haji Akber; Ito Yoichiro
2011
Source PublicationJournal of Separation Science
ISSN16159306
Volume34Issue:10Pages:1205-1211
AbstractThree types of novel coiled column configurations, i.e. a triangular coiled column and elliptical coiled columns I and II, were designed for type-I countercurrent chromatography and their performances were evaluated with two solvent systems each with suitable test samples. Three dinitrophenyl (DNP) amino acids (DNP-DL-glu, DNP-β-ala and DNP-L-ala) were separated with a moderately hydrophobic two-phase solvent system composed of hexane-ethyl acetate-metanol-0.1 M hydrochloric acid (1:1:1:1, v/v), while two dipeptides (tryptophyl-tyrosine and valyl-tyrosine) were separated with a polar solvent system composed of 1-butanol-acetic acid-water (4.75:0.25:5, v/v). The overall results indicated that the performance of compact type-I countercurrent chromatography was improved by elliptical coiled column I which was mounted with its maximum coil diameter perpendicular to the surface of the column holder. Hydrodynamic effects involved in these separations were discussed. Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
KeywordCoiled Column Configuration Compact Type-i Countercurrent Chromatography Dinitrophenyl Amino Acid Dipeptide Retention Of The Stationary Phase Resolution
Subject AreaChemistry
DOI10.1002/jssc.201100019
WOS IDWOS:000290635400016
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Document Type期刊论文
Identifierhttp://ir.xjipc.cas.cn/handle/365002/1446
Collection省部共建新疆特有药用资源利用重点实验室
AffiliationNHLBI, Bioseparat Technol Lab, Biochem & Biophys Ctr, NIH, Bethesda, MD 20892 USA;Chinese Acad Sci, Key Lab Xinjiang Indigenous Med Plants Resource U, Xinjiang Tech Inst Phys & Chem, Urumqi, Peoples R China
Recommended Citation
GB/T 7714
Yang Yi,Gu Dongyu,Aisa Haji Akber,et al. studies on the performance of different coiled column configurations for compact type-i countercurrent chromatography[J]. Journal of Separation Science,2011,34(10):1205-1211.
APA Yang Yi,Gu Dongyu,Aisa Haji Akber,&Ito Yoichiro.(2011).studies on the performance of different coiled column configurations for compact type-i countercurrent chromatography.Journal of Separation Science,34(10),1205-1211.
MLA Yang Yi,et al."studies on the performance of different coiled column configurations for compact type-i countercurrent chromatography".Journal of Separation Science 34.10(2011):1205-1211.
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