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Biotechnological production of eicosapentaenoic acid: From a metabolic engineering point of view
Cao Yujin; Cao Yugang; Min’an Zhao
Source PublicationProcess Biochemistry

Eicosapentaenoic acid (EPA) is an ω3 polyunsaturated fatty acid which has been demonstrated to play important roles in a number of aspects of human health. EPA is traditionally obtained from marine fish oils. However, the shrinking fish populations are making the sustainability of these sources questionable. Consequently, alternative sources of EPA are being sought, especially from marine microalgae, bacteria, and fungi. These microorganisms contain relatively large amounts of high-quality EPA and they are the primary producers of this important fatty acid. There are two distinct pathways for EPA de novo biosynthesis in microbial systems: the desaturation and elongation pathway and the polyketide pathway. Genes involved in the biosynthetic pathways have been identified from different microorganisms and characterized in depth. In addition, numerous strategies have been developed for commercial production of EPA by microbial fermentation, among which strain improvements by genetic engineering could provide high-yield producers of EPA. In this review, we summarize recent efforts and experiences devoted to metabolic engineering of various microorganisms that lead to efficient biocatalysts for the production of EPA, as well as the key limitations and challenges. The combination of traditional biochemistry and molecular biology with new systems biology and synthetic biology tools will provide a better view of EPA biosynthesis and a greater potential of microbial production. Continued advances in metabolic engineering will help to improve the final titer, productivity, and yield of EPA.

KeywordBiosynthesis Eicosapentaenoic Acid Metabolic Engineering Polyunsaturated Fatty Acid
Subject AreaBiochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Engineering
WOS IDWOS:000306777400004
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Cited Times:13[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
AffiliationChinese Acad Sci, Xinjiang Tech Inst Phys&Chem, Xinjiang Key Lab Plant Resources & Nat Prod Chem, Urumqi 830011, Peoples R China
Recommended Citation
GB/T 7714
Cao Yujin,Cao Yugang,Min’an Zhao. Biotechnological production of eicosapentaenoic acid: From a metabolic engineering point of view[J]. Process Biochemistry,2012,47(9):1320-1326.
APA Cao Yujin,Cao Yugang,&Min’an Zhao.(2012).Biotechnological production of eicosapentaenoic acid: From a metabolic engineering point of view.Process Biochemistry,47(9),1320-1326.
MLA Cao Yujin,et al."Biotechnological production of eicosapentaenoic acid: From a metabolic engineering point of view".Process Biochemistry 47.9(2012):1320-1326.
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