Biotechnological production of eicosapentaenoic acid: From a metabolic engineering point of view
Cao Yujin; Cao Yugang; Min’an Zhao
2012
发表期刊Process Biochemistry
ISSN1359-5113
卷号47期号:9页码:1320-1326
摘要

Eicosapentaenoic acid (EPA) is an ω3 polyunsaturated fatty acid which has been demonstrated to play important roles in a number of aspects of human health. EPA is traditionally obtained from marine fish oils. However, the shrinking fish populations are making the sustainability of these sources questionable. Consequently, alternative sources of EPA are being sought, especially from marine microalgae, bacteria, and fungi. These microorganisms contain relatively large amounts of high-quality EPA and they are the primary producers of this important fatty acid. There are two distinct pathways for EPA de novo biosynthesis in microbial systems: the desaturation and elongation pathway and the polyketide pathway. Genes involved in the biosynthetic pathways have been identified from different microorganisms and characterized in depth. In addition, numerous strategies have been developed for commercial production of EPA by microbial fermentation, among which strain improvements by genetic engineering could provide high-yield producers of EPA. In this review, we summarize recent efforts and experiences devoted to metabolic engineering of various microorganisms that lead to efficient biocatalysts for the production of EPA, as well as the key limitations and challenges. The combination of traditional biochemistry and molecular biology with new systems biology and synthetic biology tools will provide a better view of EPA biosynthesis and a greater potential of microbial production. Continued advances in metabolic engineering will help to improve the final titer, productivity, and yield of EPA.

关键词Biosynthesis Eicosapentaenoic Acid Metabolic Engineering Polyunsaturated Fatty Acid
学科领域Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Engineering
DOI10.1016/j.procbio.2012.05.011
WOS记录号WOS:000306777400004
引用统计
被引频次:13[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.xjipc.cas.cn/handle/365002/1401
专题省部共建新疆特有药用资源利用重点实验室
作者单位Chinese Acad Sci, Xinjiang Tech Inst Phys&Chem, Xinjiang Key Lab Plant Resources & Nat Prod Chem, Urumqi 830011, Peoples R China
推荐引用方式
GB/T 7714
Cao Yujin,Cao Yugang,Min’an Zhao. Biotechnological production of eicosapentaenoic acid: From a metabolic engineering point of view[J]. Process Biochemistry,2012,47(9):1320-1326.
APA Cao Yujin,Cao Yugang,&Min’an Zhao.(2012).Biotechnological production of eicosapentaenoic acid: From a metabolic engineering point of view.Process Biochemistry,47(9),1320-1326.
MLA Cao Yujin,et al."Biotechnological production of eicosapentaenoic acid: From a metabolic engineering point of view".Process Biochemistry 47.9(2012):1320-1326.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
biotechnological pro(507KB)期刊论文作者接受稿开放获取CC BY-NC-SA浏览 请求全文
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Cao Yujin]的文章
[Cao Yugang]的文章
[Min’an Zhao]的文章
百度学术
百度学术中相似的文章
[Cao Yujin]的文章
[Cao Yugang]的文章
[Min’an Zhao]的文章
必应学术
必应学术中相似的文章
[Cao Yujin]的文章
[Cao Yugang]的文章
[Min’an Zhao]的文章
相关权益政策
暂无数据
收藏/分享
文件名: biotechnological production of eicosapentaenoic acid from a metabolic engineering point of view.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。