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Bioassay-guided isolation of antioxidants from Astragalus altaicus by combination of chromatographic techniques
Yang Chunyan; Yang Yi; Aisa Haji Akber; Xin Xuelei; Ma Hairong; Yili Abulimiti; Zhao Yongxin
2012
Source PublicationJournal of Separation Science
ISSN16159306
Volume35Issue:8Pages:977-983
AbstractAn efficient method for bioassay-guided preparative isolation of antioxidants from the n-butanol extract of Astragalus altaicus Bunge was ingeniously developed by combination of silica gel column chromatography and high-speed counter-current chromatography. Under the bioassay-guidance of antioxidant activities, the antioxidants were gradually separated from the crude sample of Astragalus altaicus Bunge by silica gel column chromatography and high-speed counter-current chromatography. Silica gel column chromatography separation was performed with chloroform, chloroform-methanol (100:1∼5:1, v/v) and chloroform-methanol-water (5:1:0.1∼2:1:0.1, v/v/v). High-speed counter-current chromatography separation was performed with a two-phase solvent system composed of ethyl acetate-n-butanol-water (2:1:6, v/v/v), which was successfully selected by thin layer chromatography analysis, at a flow rate of 1.5 mL/min. As a result, isorhamnetin-3-gentiobioside (20.8 mg), rutin (82.0 mg), and narcissin (12.8 mg) were obtained for the first time from 200 mg of the crude sample, ABS-5 of Astragalus altaicus Bunge. The purities were all at over 95% by high-performance liquid chromatography analysis, and their structures were unambiguously identified by mass spectroscopy, 1H, and 13C nuclear magnetic resonance spectroscopy. Antioxidant activities of the three compounds were also assayed by in vitro ABTS [2,2'-azino-bis-(3- ethylbenzothiazoline-6-sulphonic acid) diamonium salt] radical cation scavenging activity. Among them, rutin possessed the highest antioxidant capacity with SC50 value of 22.15 μg/mL. © 2012 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.
KeywordAntioxidants Astragalus Altaicus Bunge Bioassay-guided Isolation Combination Of Chromatographic Techniques Flavonoids High-speed Counter-current Chromatography
Subject AreaChemistry
DOI10.1002/jssc.201101104
WOS IDWOS:000304038200009
Citation statistics
Cited Times:17[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.xjipc.cas.cn/handle/365002/1398
Collection省部共建新疆特有药用资源利用重点实验室
资源化学研究室
AffiliationChinese Acad Sci, Xinjiang Tech Inst Phys & Chem, Key Lab Xinjiang Indigenous Med Plants Resource U, Urumqi 830011, Peoples R China;China Three Gorges Univ, Coll Med, Yichang, Peoples R China
Recommended Citation
GB/T 7714
Yang Chunyan,Yang Yi,Aisa Haji Akber,et al. Bioassay-guided isolation of antioxidants from Astragalus altaicus by combination of chromatographic techniques[J]. Journal of Separation Science,2012,35(8):977-983.
APA Yang Chunyan.,Yang Yi.,Aisa Haji Akber.,Xin Xuelei.,Ma Hairong.,...&Zhao Yongxin.(2012).Bioassay-guided isolation of antioxidants from Astragalus altaicus by combination of chromatographic techniques.Journal of Separation Science,35(8),977-983.
MLA Yang Chunyan,et al."Bioassay-guided isolation of antioxidants from Astragalus altaicus by combination of chromatographic techniques".Journal of Separation Science 35.8(2012):977-983.
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