Bioassay-guided isolation of antioxidants from Astragalus altaicus by combination of chromatographic techniques
Yang Chunyan; Yang Yi; Aisa Haji Akber; Xin Xuelei; Ma Hairong; Yili Abulimiti; Zhao Yongxin
2012
发表期刊Journal of Separation Science
ISSN16159306
卷号35期号:8页码:977-983
摘要An efficient method for bioassay-guided preparative isolation of antioxidants from the n-butanol extract of Astragalus altaicus Bunge was ingeniously developed by combination of silica gel column chromatography and high-speed counter-current chromatography. Under the bioassay-guidance of antioxidant activities, the antioxidants were gradually separated from the crude sample of Astragalus altaicus Bunge by silica gel column chromatography and high-speed counter-current chromatography. Silica gel column chromatography separation was performed with chloroform, chloroform-methanol (100:1∼5:1, v/v) and chloroform-methanol-water (5:1:0.1∼2:1:0.1, v/v/v). High-speed counter-current chromatography separation was performed with a two-phase solvent system composed of ethyl acetate-n-butanol-water (2:1:6, v/v/v), which was successfully selected by thin layer chromatography analysis, at a flow rate of 1.5 mL/min. As a result, isorhamnetin-3-gentiobioside (20.8 mg), rutin (82.0 mg), and narcissin (12.8 mg) were obtained for the first time from 200 mg of the crude sample, ABS-5 of Astragalus altaicus Bunge. The purities were all at over 95% by high-performance liquid chromatography analysis, and their structures were unambiguously identified by mass spectroscopy, 1H, and 13C nuclear magnetic resonance spectroscopy. Antioxidant activities of the three compounds were also assayed by in vitro ABTS [2,2'-azino-bis-(3- ethylbenzothiazoline-6-sulphonic acid) diamonium salt] radical cation scavenging activity. Among them, rutin possessed the highest antioxidant capacity with SC50 value of 22.15 μg/mL. © 2012 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.
关键词Antioxidants Astragalus Altaicus Bunge Bioassay-guided Isolation Combination Of Chromatographic Techniques Flavonoids High-speed Counter-current Chromatography
学科领域Chemistry
DOI10.1002/jssc.201101104
WOS记录号WOS:000304038200009
引用统计
文献类型期刊论文
条目标识符http://ir.xjipc.cas.cn/handle/365002/1398
专题省部共建新疆特有药用资源利用重点实验室
作者单位Chinese Acad Sci, Xinjiang Tech Inst Phys & Chem, Key Lab Xinjiang Indigenous Med Plants Resource U, Urumqi 830011, Peoples R China;China Three Gorges Univ, Coll Med, Yichang, Peoples R China
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Yang Chunyan,Yang Yi,Aisa Haji Akber,et al. Bioassay-guided isolation of antioxidants from Astragalus altaicus by combination of chromatographic techniques[J]. Journal of Separation Science,2012,35(8):977-983.
APA Yang Chunyan.,Yang Yi.,Aisa Haji Akber.,Xin Xuelei.,Ma Hairong.,...&Zhao Yongxin.(2012).Bioassay-guided isolation of antioxidants from Astragalus altaicus by combination of chromatographic techniques.Journal of Separation Science,35(8),977-983.
MLA Yang Chunyan,et al."Bioassay-guided isolation of antioxidants from Astragalus altaicus by combination of chromatographic techniques".Journal of Separation Science 35.8(2012):977-983.
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