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牛乳铁蛋白基因N-lobe的cDNA克隆及毕赤酵母表达载体的构建
Alternative Titlecdna cloning of bovine lactoferrin n-lobe gene and construction of pichia pastoris expression vector
林松涛; 张居农; 王亮; 史芳芳; 李卫华; 张钰; 吕自力; 王安江
2008
Source Publication家畜生态学报
ISSN1673-1182
Volume29Issue:5Pages:13-18
Abstract以牛乳腺组织总RAN为模板,经RT-PCR得到牛乳铁蛋白基因的N-lobe片段并将其克隆到pGM-T克隆载体上,最后将其连入表达载体pPICZαA中,构建牛乳铁蛋白基因N-lobe的酵母表达载体。结果表明:克隆片段大小为1028bp,与Gen Bank中登录的序列的相应部位相比,所获牛乳铁蛋白N-lobe cDNA序列的同源性为99.7%;重组表达质粒经酶切和PCR鉴定构建正确,为酵母表达乳铁蛋白基因N-lobe奠定了基础。
Keyword乳铁蛋白 N-lobe 表达载体
Document Type期刊论文
Identifierhttp://ir.xjipc.cas.cn/handle/365002/1350
Collection资源化学研究室
Affiliation中国科学院新疆理化技术研究所;石河子大学动物科技学院;新疆金牛生物有限公司
Recommended Citation
GB/T 7714
林松涛,张居农,王亮,等. 牛乳铁蛋白基因N-lobe的cDNA克隆及毕赤酵母表达载体的构建[J]. 家畜生态学报,2008,29(5):13-18.
APA 林松涛.,张居农.,王亮.,史芳芳.,李卫华.,...&王安江.(2008).牛乳铁蛋白基因N-lobe的cDNA克隆及毕赤酵母表达载体的构建.家畜生态学报,29(5),13-18.
MLA 林松涛,et al."牛乳铁蛋白基因N-lobe的cDNA克隆及毕赤酵母表达载体的构建".家畜生态学报 29.5(2008):13-18.
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